Diagnostic Microbiology & Infectious Disease
Volume 45, Issue 1 , Pages 53-61 , January 2003

Detection and drug-susceptibility testing of M. tuberculosis from sputum samples using luciferase reporter phage: comparison with the Mycobacteria Growth Indicator Tube (MGIT) system

  • Svetoslav Bardarov Jr.

      Affiliations

    • Albert Einstein College of Medicine, Bronx, NY, USA
    • Contributed equally to this manuscript.
  • ,
  • Horng Dou

      Affiliations

    • Albert Einstein College of Medicine, Bronx, NY, USA
    • Contributed equally to this manuscript.
  • ,
  • Katherine Eisenach

      Affiliations

    • University of Arkansas Medical Center, Little Rock, AK, USA
  • ,
  • Niaz Banaiee

      Affiliations

    • Stanford University, Palo Alto, CA, USA
  • ,
  • S.u Ya

      Affiliations

    • Albert Einstein College of Medicine, Bronx, NY, USA
  • ,
  • John Chan

      Affiliations

    • Albert Einstein College of Medicine, Bronx, NY, USA
  • ,
  • William R. Jacobs Jr

      Affiliations

    • Albert Einstein College of Medicine, Bronx, NY, USA
  • ,
  • Paul F. Riska

      Affiliations

    • State University of New York-Downstate Medical Center, Brooklyn, NY, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-718-270-4181; fax: +1-718-270-4123.

Received 11 April 2002 ,Accepted 19 August 2002.

References 

  1. Alcaide F, Benitez MA, Escriba JM, Martin R. Evaluation of the BACTEC MGIT 960 and the MB/BacT systems for recovery of mycobacteria from clinical specimens and for species identification by DNA AccuProbe. J Clin Microbiol. 2000;38:398–401
  2. Banaiee N, Bobadilla-Del-Valle M, Bardarov S, Riska PF, Small PM, Ponce-De-Leon A, et al. Luciferase reporter mycobacteriophages for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico. J Clin Microbiol. 2001;39:3883–3888
  3. Bemer P, Palicova F, Rusch-Gerdes S, Drugeon HB, Pfyffer GE. Multicenter evaluation of fully automated BACTEC Mycobacteria Growth Indicator Tube 960 system for susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol. 2002;40:150–154
  4. Bird BR, Denniston MM, Huebner RE, Good RC. Changing Practices in Mycobacteriology (a Follow-up Survey of State and Territorial Public Health Laboratories). J Clin Microbiol. 1996;34:554–559
  5. Brown KL, Sarkis GJ, Wadsworth C, Hatfull GF. Transcriptional silencing by the mycobacteriophage L5 repressor. EMBO J. 1997;16:5914–5921
  6. Carriere C., Riska P. F., Zimhony O., Kriakov J., Bardarov S., Burns J., Chan J., & Jacobs W. R., Jr. (1997). Conditionally replicating luciferase reporter phages: improved sensitivity for rapid detection and assessment of drug susceptibility of Mycobacterium tuberculosis. J Clin Microbiol 35, 3232–3239.
  7. Caviedes L, Lee TS, Gilman RH, Sheen P, Spellman E, Lee EH, et al. Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru. J Clin Microbiol. 2000;38:1203–1208
  8. Edlin BR, Tokars JI, Grieco MH, Crawford JT, Williams J, Sordillo EM, et al  An outbreak of multidrug-resistant tuberculosis among hospitalized patients with the acquired immunodeficiency syndrome. [see comments] New Engl J Med. 1992;326:1514–1521
  9. Espinal MA, Kim SJ, Suarez PG, Kam KM, Khomenko AG, Migliori GB, et al. Standard short-course chemotherapy for drug-resistant tuberculosis (treatment outcomes in 6 countries). [see comments] JAMA. 2000;283:2537–2545
  10. Espinal MA, Laszlo A, Simonsen L, Boulahbal F, Kim SJ, Reniero A, et al. Global trends in resistance to antituberculosis drugs. World Health Organization-International Union against Tuberculosis and Lung Disease Working Group on Anti-Tuberculosis Drug Resistance Surveillance. New Engl J Med. 2001;344:1294–1303
  11. Hadgu A. Discrepant analysis is an inappropriate and unscientific method. J Clin Microbiol. 2000;38:4301–4302
  12. Heifets LB, Cangelosi GA. Drug susceptibility testing of Mycobacterium tuberculosis (a neglected problem at the turn of the century). [see comments] Int J Tuberc Lung Dis. 1999;3:564–581
  13. Heifets LB, Good RC. Current Laboratory Methods for the Diagnosis of Tuberculosis. In:  Bloom BR editors. Tuberculosis:Pathogenesis, Protection and Control. Washington DC: ASM Press; 1994;p. 85–110
  14. Hobby GL, Holman AP, Iseman MD, Jones JM. Enumeration of tubercle bacilli in sputum of patients with pulmonary tuberculosis. Antimicrob Agents Chemother. 1973;4:94–104
  15. Jacobs WR, Barletta RG, Udani R, Chan J, Kalkut G, Sosne G, et al. Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science. 1993;260:819–822
  16. Kent PT, Kubica GP. Public Health Mycobacteriology (a Guide for the Level III Laboratory). Atlanta: Centers for Disease Control; 1985;
  17. Mejia GI, Castrillon L, Trujillo H, Robledo JA. Microcolony detection in 7H11 thin layer culture is an alternative for rapid diagnosis of Mycobacterium tuberculosis infection. [see comments] Int J Tuberc Lung Dis. 1999;3:138–142
  18. Pablos-Mendez A., Raviglione M. C., Laszlo A., Binkin N., Rieder H. L., Bustreo F., Cohn D. L., Lambregts-van Weezenbeek C. S., Kim S. J., Chaulet P., & Nunn P. (1998). Global surveillance for antituberculosis-drug resistance, 1994–1997. World Health Organization-International Union against Tuberculosis and Lung Disease Working Group on Anti-Tuberculosis Drug Resistance Surveillance. New Engl J Med 338, 1641–1649
  19. Raviglione M. C., Snider D. E., Jr., & Kochi A. (1995). Global Epidemiology of Tuberculosis: Morbidity and Mortality of a Worldwide Epidemic. JAMA 273, 220–226.
  20. Riska P. F., & Jacobs W. R., Jr. (1998). The use of luciferase-reporter phage for antibiotic-susceptibility testing of mycobacteria. Methods Mol Biol 101, 431–455
  21. Riska P. F., Jacobs W. R., Jr., & Alland D. (2000). Molecular determinants of drug resistance in tuberculosis. Int J Tuberc Lung Dis 4, S4–10.
  22. Riska P. F., Jacobs W. R., Jr., Bloom B. R., McKitrick J., & Chan J. (1997). Specific identification of Mycobacterium tuberculosis with the luciferase reporter mycobacteriophage: use of p-nitro-α- acetylamino-β-hydroxy propiophenone. J Clin Microbiol 35, 3225–3231
  23. Riska P. F., Su Y., Bardarov S., Freundlich L., Sarkis G., Hatfull G., Carriere C., Kumar V., Chan J., & Jacobs W. R., Jr. (1999). Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx Box. J Clin Microbiol 37, 1144–1149
  24. Rusch-Gerdes S, Domehl C, Nardi G, Gismondo MR, Welscher HM, Pfyffer GE. Multicenter evaluation of the mycobacteria growth indicator tube for testing susceptibility of Mycobacterium tuberculosis to first-line drugs. J Clin Microbiol. 1999;37:45–48
  25. Siddiqi S. H. (1995). BACTEC TB Product and Procedure Manual, Revision D, D edn. Becton Dickinson, Sparks, MD
  26. Tortoli E, Benedetti M, Fontanelli A, Simonetti MT. Evaluation of automated BACTEC MGIT 960 system for testing susceptibility of mycobacterium tuberculosis to four major antituberculous drugs (comparison with the radiometric BACTEC 460TB method and the agar plate method of proportion). J Clin Microbiol. 2002;40:607–610
  27. Turett GS, Telzak EE, Torian LV, Blum S, Alland D, Weisfuse I, et al. Improved Outcomes for Patients with Multidrug-Resistant Tuberculosis. Clin Infect Dis. 1995;21:1238–1244
  28. Wallis RS, Perkins MD, Phillips M, Joloba M, Namale A, Johnson JL, et al. Predicting the outcome of therapy for pulmonary tuberculosis. Am J Respir Crit Care Med. 2000;161:1076–1080

PII: S0732-8893(02)00478-9

doi: 10.1016/S0732-8893(02)00478-9

Diagnostic Microbiology & Infectious Disease
Volume 45, Issue 1 , Pages 53-61 , January 2003