Diagnostic Microbiology & Infectious Disease
Volume 45, Issue 1 , Pages 29-34 , January 2003

Comparison of BDPhoenix and VITEK2 automated antimicrobial susceptibility test systems for extended-spectrum beta-lactamase detection in Escherichia coli and Klebsiella species clinical isolates

  • Enno Stürenburg

      Affiliations

    • Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49-40-42803-3147; fax: +49-40-42803-4881.
    • ,
  • Ingo Sobottka

      Affiliations

    • Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany
    • ,
  • Heinz-Hubert Feucht

      Affiliations

    • Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany
    • ,
  • Dietrich Mack

      Affiliations

    • Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany
    • ,
  • Rainer Laufs

      Affiliations

    • Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany

Received 16 April 2002 ,Accepted 21 August 2002.

References 

  1. Bauernfeind A, Stemplinger I, Jungwirth R, Ernst S, Casellas JM. Sequences of β-lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other β- lactamases. Antimicrob Agents Chemother. 1996;40:509–513
  2. Bradford PA. Automated thermal cycling is superior to traditional methods for nucleotide sequencing of blaSHV genes. Antimicrob Agents Chemother. 1999;43:2960–2963
  3. Bradford PA. Extended-spectrum β-lactamases in the 21th century (characterization, epidemiology, and detection of this important resistance threat). Clin Microbiol Rev. 2001;14:933–951
  4. Canton R, Perez-Vasquez M, Oliver A, Coque TM, Loza E, Ponz F, et al. Validation of the VITEK 2 and the advanced expert system with a collection of enterobacteriaceae harboring extended spectrum or inhibitor resistant β-lactamases. Diagn Microbiol Infect Dis. 2001;41:65–70
  5. Ferraro MJ, Jorgensen JH. Susceptibility testing instrumentation and computerized expert systems for data analysis and interpretation. In:  Murray PR,  Baron EJ,  Pfaller MA,  Tenover FC,  Yolken RH editor. Manual of Clinical Microbiology. 7th Edition. Washington, DC: ASM Press; 1999;
  6. Gibb AP, Crichton M. Cefpodoxime screening of Escherichia coli and Klebsiella spp. by Vitek for detection of organisms producing extended-spectrum β-lactamases. Diagn Microbiol Infect Dis. 2000;38:255–257
  7. Jorgensen JH, Ferraro MJ. Antimicrobial susceptibility testing (special needs for fastidious organisms and difficult-to-detect resistance mechanisms). Clin Infect Dis. 2000;26:973–980
  8. Kim YK, Pai H, Lee H-J, Park S-E, Choi E-H, Kim J, et al. Bloodstream infections by extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in children (epidemiology and clinical outcome). Antimicrob Agents Chemother. 2002;46:1481–1491
  9. Livermore DM. β-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev. 1995;8:557–584
  10. Moland ES, Sanders CC, Thomson KS. Can results obtained with commercially available MicroScan microdilution panels serve as an indicator of β-lactamase production among Escherichia coli and Klebsiella isolates with hidden resistance to expanded-spectrum cephalosporins and aztreonam. J Clin Microbiol. 1998;36:2575–2579
  11. NCCLS. M7-A5. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved Standard. Fifth Edition. Wayne, Pennsylvania, USA: National Committee for Clinical Laboratory Standards; 2000;
  12. NCCLS. M100-S12. Performance Standards for Antimicrobial Susceptibility Testing; Twelfth Informational Supplement. Wayne, Pennsylvania, USA: National Committee for Clinical Laboratory Standards; 2002;
  13. Paterson DL, Ko W-C, von Gottberg A, Casellas JM, Mulazimoglu L, Klugman KP, et al. Outcome of cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended-spectrum β-lactamases (implications for the clinical microbiology laboratory). J Clin Microbiol. 2001;39:2206–2212
  14. Poyart C, Mugnier P, Quesne G, Berche P, Trieu-Cuot P. A novel extended-spectrum TEM-type β-lactamase (TEM-52) associated with decreased susceptibility to moxalactam in Klebsiella pneumoniae. Antimicrob Agents Chemother. 1998;42:108–113
  15. Sanders CC, Barry AL, Washington JA, Shubert C, Moland ES, Traczewski MM, et al. Detection of extended-spectrum-β-lactamase-producing members of the family Enterobacteriaceae with Vitek ESBL test. J Clin Microbiol. 1996;34:2997–3001
  16. Sanders CC, Peyret M, Moland ES, Shubert C, Thomson KS, Boeufgras JM, et al. Ability of the VITEK 2 advanced expert system to identify β-lactam phenotypes in isolates of Enterobacteriaceae and Pseudomonas aeruginosa. J Clin Microbiol. 2000;38:570–574
  17. Sutcliffe JG. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci USA. 1978;75:3737–3741
  18. Thomson KS, Sanders CC, Moland ES. Use of microdilution panels with and without β-lactamase inhibitors as a phenotypic test for β-lactamase production among Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter freundii, and Serratia marcescens. Antimicrob Agents Chemother. 1999;43:1393–1400

PII: S0732-8893(02)00481-9

doi: 10.1016/S0732-8893(02)00481-9

Diagnostic Microbiology & Infectious Disease
Volume 45, Issue 1 , Pages 29-34 , January 2003

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