Diagnostic Microbiology & Infectious Disease
Volume 45, Issue 1 , Pages 81-84 , January 2003

Real-time PCR for the rapid detection of vanA and vanB genes

  • Silvano Palladino

      Affiliations

    • Department of Microbiology & Infectious Diseases, Royal Perth Hospital, Perth, Western Australia
    • Corresponding Author InformationCorresponding author. Tel.: +61 8 9224 2444; fax: +61 8 9224 1799.
    • ,
  • Ian D. Kay

      Affiliations

    • Department of Microbiology & Infectious Diseases, Royal Perth Hospital, Perth, Western Australia
    • ,
  • Anna Maria Costa

      Affiliations

    • Department of Microbiology & Infectious Diseases, Royal Perth Hospital, Perth, Western Australia
    • ,
  • Erica J. Lambert

      Affiliations

    • Department of Microbiology & Infectious Diseases, Royal Perth Hospital, Perth, Western Australia
    • ,
  • James P. Flexman

      Affiliations

    • Department of Microbiology & Infectious Diseases, Royal Perth Hospital, Perth, Western Australia

Received 10 May 2002 ,Accepted 26 August 2002.

References 

  1. Chen Y, Marshall SA, Winokur PL, Coffman SL, Wilke WW, Murray PR, et al. Use of molecular and reference susceptibility testing methods in a multicenter evaluation of microscan dried overnight gram-positive MIC panels for detection of vancomycin and high-level aminoglycoside resistances in enterococci. J Clin Microbiol. 1998;36:2996–3001
  2. Coombs GW, Kay ID, Steven RA, Pearman JW, Bertolatti D, Grubb WB. Should genotypic testing be done on all phenotypically vancomycin-resistant enterococci detected in hospitals. J Clin Microbiol. 1999;37:1229–1230
  3. Dutka-Malen S, Evers S, Courvalin P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol. 1995;33:24–27
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  8. Jayaratne P, Rutherford C. Detection of clinically relevant genotypes of vancomycin-resistant enterococci in nosocomial surveillance specimens by PCR. J Clin Microbiol. 1999;37:2090–2092
  9. Jones RN, Marshall SA, Pfaller MA, Wilke WW, Hollis RJ, Erwin ME, et al. Nosocomial enterococcal blood stream infections in the SCOPE program (antimicrobial resistance, species occurrence, molecular testing results, and laboratory testing accuracy). Diagn Microbiol Infect Dis. 1997;29:95–102
  10. National Committee for Clinical Laboratory Standards.. Molecular diagnostic methods for infectious diseases; approved guideline. MM3-A. Wayne, Pa: National Committee for Clinical Laboratory Standards; 1995;
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  12. Patel R, Uhl JR, Kohner P, Hopkins MK, Cockerill FR. Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci. J Clin Microbiol. 1997;35:703–707
  13. Pérez-Hernandez X, Mendez-Alvarez S, Claverie-Martin F. A PCR assay for rapid detection of vancomycin-resistant enterococci. Diagn Microbiol Infect Dis. 2002;42(4):273–277

PII: S0732-8893(02)00505-9

doi: 10.1016/S0732-8893(02)00505-9

Diagnostic Microbiology & Infectious Disease
Volume 45, Issue 1 , Pages 81-84 , January 2003

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