Diagnostic Microbiology & Infectious Disease
Volume 54, Issue 1 , Pages 73-77 , January 2006

Etest synergy testing of clinical isolates of Staphylococcus aureus demonstrating heterogeneous resistance to vancomycin

  • Brian T. Tsuji

      Affiliations

    • Anti-Infective Research Laboratory, Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA
    • Department of Pharmacy Services, Detroit Receiving Hospital and University Health Center, Detroit, MI 48201, USA
    • Current address: School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14260, USA.
    • ,
  • Michael J. Rybak

      Affiliations

    • Anti-Infective Research Laboratory, Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA
    • Department of Pharmacy Services, Detroit Receiving Hospital and University Health Center, Detroit, MI 48201, USA
    • School of Medicine, Wayne State University, Detroit, MI 48201, USA
    • Corresponding Author InformationCorresponding author. Anti-Infective Research Laboratory, Department of Pharmacy Practice 4148, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI 48201. Tel.: +1-313-993-4673; fax: +1-313-577-8915.

Received 14 June 2005 ,Revised 26 August 2005 ,Accepted 29 August 2005.

References 

  1. Ariza J, Pujol M, Cabo J, Pena C, Fernandez N, Linares J, et al. Vancomycin in surgical infections due to methicillin-resistant Staphylococcus aureus with heterogeneous resistance to vancomycin. Lancet. 1999;353:1587–1588
  2. Bolstrum A, Arvdvison S, Ericson M, Karlsonn A. New in vitro models for studying antibiotic combination using predefined antibiotics gradient. In: Progam and abstracts of the 16th International Congress of Chemotherapy, Jerusalem, Abstract No. 11. 1989;
  3. Bonapace CR, White RL, Friedrich LV, Bosso JA. Evaluation of antibiotic synergy against Acinetobacter baumannii: a comparison with Etest, time-kill, and checkerboard methods. Diagn. Microbiol. Infect. Dis. 2000;38:43–50
  4. Bonapace CR, Bosso JA, Friedrich LV, White RL. Comparison of methods of interpretation of checkerboard synergy testing. Diagn. Microbiol. Infect. Dis. 2002;44:363–366
  5. Cappelletty DM, Rybak MJ. Comparison of methodologies for synergism testing of drug combinations against resistant strains of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 1996;40:677–683
  6. Eguia JM, Liu C, Moore M, Wrone EM, Pont J, Gerberding JL, et al. Low colonization prevalence of Staphylococcus aureus with reduced vancomycin susceptibility among patients undergoing hemodialysis in the San Francisco Bay area. Clin. Infect. Dis. 2005;1617–1624
  7. Fridkin SK, Hageman J, McDougal LK, Mohammed J, Jarvis WR, Perl TM, et al. Epidemiological and microbiological characterization of infections caused by Staphylococcus aureus with reduced susceptibility to vancomycin, United States, 1997–2001. Clin. Infect. Dis. 2003;429–439
  8. Hiramatsu K, Aritaka N, Hanaki H, Kawasaki S, Hosoda Y, Hori S, et al. Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin. Lancet. 1997;350:70–73
  9. Howden BP, Ward PB, Charles PGP, Korman TM, Fuller A, du Cros P, et al. Treatment outcomes for serious infections caused by methicillin-resistant Staphylococcus aureus with reduced vancomycin susceptibility. Clin. Infect. Dis. 2004;38:521–528
  10. Hanberger H, Nilsson LE, Maller R, Isaksson B. Pharmacodynamics of daptomycin and vancomycin on Enterococcus faecalis and Staphylococcus aureus demonstrated by studies of initial killing and postantibiotic effect and influence of Ca2+ and albumin on these drugs. Antimicrob. Agents Chemother. 1991;35:1710–1716
  11. Lamp KC, Rybak MJ. Teicoplanin and daptomycin bactericidal activities in the presence of albumin or serum under controlled conditions of pH and ionized calcium. Antimicrob. Agents Chemother. 1993;37:605–609
  12. Lewis RE, Diekema DJ, Messer SA, Pfaller MA, Klepser ME. Comparison of Etest, chequerboard dilution and time-kill studies for the detection of synergy or antagonism between antifungal agents tested against Candida species. J. Antimicrob. Chemother. 2002;345–351
  13. Liu C, Chambers HF. Staphylococcus aureus with heterogeneous resistance to vancomycin: epidemiology, clinical significance, and critical assessment of diagnostic methods. Antimicrob. Agents Chemother. 2003;47:3040–3045
  14. Manno G, Ugolotti E, Belli ML, Fenu ML, Romano L, Cruciani M. Use of the E test to assess synergy of antibiotic combinations against isolates of Burkholderia cepacia-complex from patients with cystic fibrosis. Eur. J. Clin. Microbiol. Infect. Dis. 2003;28–34
  15. Moore MR, Perdreau-Remington F, Chambers HF. Vancomycin treatment failure associated with heterogeneous vancomycin-intermediate Staphylococcus aureus in a patient with endocarditis and in the rabbit model of endocarditis. Antimicrob. Agents Chemother. 2003;47:1262–1266
  16. National Committee for Clinical Laboratory Standards . Performance standard for antimicrobial susceptibility testing. Document M100-S15 Wayne (PA): NCCLS; 2005;
  17. Orhan G, Bayram A, Zer Y, Balci I. Synergy tests by E test and checkerboard methods of antimicrobial combinations against Brucella melitensis. J. Clin. Microbiol. 2005;140–143
  18. Pankey GA, Ashcraft D, Prakash O. Etest synergy testing of oxacillin-resistant Staphylococcus aureus (ORSA) with linezolid (L) and moxifloxacin (M). (Abstract: E-1133) In: 42nd ICAAC Abstracts, American Society for Microbiology, September 27–30, 2002, San Diego, CA. 2002;p. 158
  19. Rybak MJ, Cha R, Cheung CM, Meka VG, Kaatz GW. Clinical isolates of Staphylococcus aureus from 1987 and 1989 demonstrating heterogeneous resistance to vancomycin and teicoplanin. Diagn. Microbiol. Infect. Dis. 2005;119–125
  20. White RL, Burgess DS, Manduru M, Bosso JA. Comparison of three different in vitro methods of detecting synergy: time-kill, checkerboard, and E test. Antimicrob. Agents Chemother. 1996;40:1914–1918

☆ A portion of this work was presented at the 44th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington, DC, October 2004.

PII: S0732-8893(05)00207-5

doi: 10.1016/j.diagmicrobio.2005.08.014

Diagnostic Microbiology & Infectious Disease
Volume 54, Issue 1 , Pages 73-77 , January 2006

Article Tools

* Image Usage & Resolution