Diagnostic Microbiology & Infectious Disease
Volume 54, Issue 1 , Pages 7-12 , January 2006

Novel enzyme immunoassay utilizing lipopolysaccharide-binding protein as a capture molecule for the measurement of chlamydial lipopolysaccharide in serum

  • Terttu Tiirola

      Affiliations

    • Department of Molecular Medicine, National Public Health Institute, Biomedicum, PO Box 104, FIN-00251 Helsinki, Finland
    • Corresponding Author InformationCorresponding author. Fax: +358-9-4744-8960.
    • ,
  • Anne Jaakkola

      Affiliations

    • Laboratory for Chlamydia and Respiratory Tract Bacteria, National Public Health Institute, PO Box 310, FIN-90101 Oulu, Finland
    • ,
  • Aini Bloigu

      Affiliations

    • Laboratory for Chlamydia and Respiratory Tract Bacteria, National Public Health Institute, PO Box 310, FIN-90101 Oulu, Finland
    • ,
  • Mika Paldanius

      Affiliations

    • Laboratory for Chlamydia and Respiratory Tract Bacteria, National Public Health Institute, PO Box 310, FIN-90101 Oulu, Finland
    • ,
  • Juha Sinisalo

      Affiliations

    • Division of Cardiology, Department of Medicine, Helsinki University Central Hospital, PO Box 340, FIN-00290 Helsinki, Finland
    • ,
  • Markku S. Nieminen

      Affiliations

    • Division of Cardiology, Department of Medicine, Helsinki University Central Hospital, PO Box 340, FIN-00290 Helsinki, Finland
    • ,
  • Sylvi Silvennoinen-Kassinen

      Affiliations

    • Department of Medical Microbiology, University of Oulu, PO Box 5000, FIN-90014 Oulu, Finland
    • ,
  • Pekka Saikku

      Affiliations

    • Department of Medical Microbiology, University of Oulu, PO Box 5000, FIN-90014 Oulu, Finland
    • ,
  • Matti Jauhiainen

      Affiliations

    • Department of Molecular Medicine, National Public Health Institute, Biomedicum, PO Box 104, FIN-00251 Helsinki, Finland
    • ,
  • Maija Leinonen

      Affiliations

    • Laboratory for Chlamydia and Respiratory Tract Bacteria, National Public Health Institute, PO Box 310, FIN-90101 Oulu, Finland

Received 12 April 2005

References 

  1. Airenne S, Surcel HM, Alakärppä H, Laitinen K, Paavonen J, Saikku P, et al. Chlamydia pneumoniae infection in human monocytes. Infect. Immun. 1999;67(3):1445–1449
  2. Boman J, Soderberg S, Forsberg J, Birgander LS, Allard A, Persson K, et al. High prevalence of Chlamydia pneumoniae DNA in peripheral blood mononuclear cells in patients with cardiovascular disease and in middle-aged blood donors. J. Infect. Dis. 1998;178:274–277
  3. Cohen J. The detection and interpretation of endotoxaemia. Intensive Care Med. 2000;26(Suppl 1):S51–S56
  4. Danesh J, Whincup P, Walker M, Lennon L, Thomson A, Appleby P, et al. Chlamydia pneumoniae IgG titres and coronary heart disease: prospective study and meta-analysis. BMJ. 2000;321:208–213
  5. Din ZZ, Mukerjee P, Kastowsky M, Takayama K. Effect of pH on solubility and ionic state of lipopolysaccharide obtained from the deep rough mutant of Escherichia coli. Biochemistry. 1993;32:4579–4586
  6. Domeika K, Brade L, Mardh PA, Brade H, Witkin SS, Domeika M. Characterization of serum antibody response to chlamydiae in patients with sexually acquired reactive arthritis. FEMS Immunol. Med. Microbiol. 1997;19:191–202
  7. Friedman MG, Dvoskin B, Kahane S. Infections with the chlamydia-like microorganism Simkania negevensis, a possible emerging pathogen. Microbes Infect. 2003;5:1013–1021
  8. Hahn DL, Dodge RW, Golubjatnikov R. Association of Chlamydia pneumoniae (strain TWAR) infection with wheezing, asthmatic bronchitis, and adult-onset asthma. JAMA. 1991;266:225–230
  9. Hailman E, Lichenstein HS, Wurfel MM, Miller DS, Johnson DA, Kelley M, et al. Lipopolysaccharide (LPS)-binding protein accelerates the binding of LPS to CD14. J. Exp. Med. 1994;179:269–277
  10. Huittinen T, Leinonen M, Tenkanen L, Mänttäri M, Virkkunen H, Pitkänen T, et al. Autoimmunity to human heat shock protein 60, Chlamydia pneumoniae infection, and inflammation in predicting coronary risk. Arterioscler. Thromb. Vasc. Biol. 2002;22:431–437
  11. Jorgensen JH, Smith RF. Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay. Appl. Microbiol. 1973;26:43–48
  12. Lee KK, Ellis AE. Rapid and sensitive silver-lipopolysaccharide staining using PhastSystem in fast horizontal polyacrylamide gel electrophoresis. Electrophoresis. 1989;10:729–731
  13. Leinonen M, Saikku P. Evidence for infectious agents in cardiovascular disease and atherosclerosis. Lancet Infect. Dis. 2002;2:11–17
  14. Leinonen M, Linnanmäki E, Mattila K, Nieminen MS, Valtonen V, Leirisalo-Repo M, et al. Circulating immune complexes containing chlamydial lipopolysaccharide in acute myocardial infarction. Microb. Pathog. 1990;9:67–73
  15. Linnanmäki E, Leinonen M, Mattila K, Nieminen MS, Valtonen V, Saikku P. Chlamydia pneumoniae-specific circulating immune complexes in patients with chronic coronary heart disease. Circulation. 1993;87:1130–1134
  16. Maass M, Jahn J, Gieffers J, Dalhoff K, Katus HA, Solbach W. Detection of Chlamydia pneumoniae within peripheral blood monocytes of patients with unstable angina or myocardial infarction. J. Infect. Dis. 2000;181(Suppl 3):S449–S451
  17. Maurin M, Eb F, Etienne J, Raoult D. Serological cross-reactions between Bartonella and Chlamydia species: implications for diagnosis. J. Clin. Microbiol. 1997;35:2283–2287
  18. Mukerjee P, Kastowsky M, Obst S, Takayama K. Lipopolysaccharide preparations in aqueous media: Implications for solution versus suspension. In:  Brade H,  Morrison D,  Opal S,  Vogel S editor. Endotoxin in health and disease. New York: Marcel-Dekker; 1999;p. 221–228
  19. Puolakkainen M, Linnanmäki E, Paavonen J, Leinonen M, Saikku P. Chlamydial lipopolysaccharide containing circulating immune complexes in pelvic inflammatory disease. In:  Bowie WR,  Jones RP,  Mårdh PA,  Ridgway GL,  Schachter J,  Stamm WE,  Ward ME editor. Chlamydial infections. Cambridge: Cambridge University Press; 1990;p. 319–322
  20. Richmond SJ, Stirling P. Localization of chlamydial group antigen in McCoy cell monolayers infected with Chlamydia trachomatis or Chlamydia psittaci. Infect. Immun. 1981;34:561–570
  21. Rund S, Lindner B, Brade H, Holst O. Structural analysis of the lipopolysaccharide from Chlamydia trachomatis serotype L2. J. Biol. Chem. 1999;274:16819–16824
  22. Saikku P. The epidemiology and significance of Chlamydia pneumoniae. J. Infect. 1992;25(Suppl 1):27–34
  23. Schumann RR, Leong SR, Flaggs GW, Gray PW, Wright SD, Mathison JC, et al. Structure and function of lipopolysaccharide binding protein. Science. 1990;249:1429–1431
  24. Sinisalo J, Mattila K, Valtonen V, Anttonen O, Juvonen J, Melin J, et al. Effect of 3 months of antimicrobial treatment with clarithromycin in acute non-q-wave coronary syndrome. Circulation. 2002;105:1555–1560
  25. Smieja M, Mahony J, Petrich A, Boman J, Chernesky M. Association of circulating Chlamydia pneumoniae DNA with cardiovascular disease: a systematic review. BMC Infect. Dis. 2002;2:21
  26. von Hertzen L, Leinonen M, Surcel HM, Karjalainen J, Saikku P. Measurement of sputum antibodies in the diagnosis of acute and chronic respiratory infections associated with Chlamydia pneumoniae. Clin. Diagn. Lab. Immunol. 1995;2:454–457
  27. Vreugdenhil AC, Snoek AM, van't Veer C, Greve JW, Buurman WA. LPS-binding protein circulates in association with apoB-containing lipoproteins and enhances endotoxin-LDL/VLDL interaction. J. Clin. Invest. 2001;107:225–234
  28. Vreugdenhil AC, Rousseau CH, Hartung T, Greve JW, van 't Veer C, Buurman WA. Lipopolysaccharide (LPS)-binding protein mediates LPS detoxification by chylomicrons. J. Immunol. 2003;170:1399–1405
  29. Waravdekar VS, Saslaw LD. A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of the malonaldehyde-thiobarbituric acid reaction. J. Biol. Chem. 1959;234:1945–1950
  30. Wong YK, Dawkins KD, Ward ME. Circulating Chlamydia pneumoniae DNA as a predictor of coronary artery disease. J. Am. Coll. Cardiol. 1999;34:1435–1439
  31. Wurfel MM, Kunitake ST, Lichenstein H, Kane JP, Wright SD. Lipopolysaccharide (LPS)-binding protein is carried on lipoproteins and acts as a cofactor in the neutralization of LPS. J. Exp. Med. 1994;180:1025–1035

PII: S0732-8893(05)00216-6

doi: 10.1016/j.diagmicrobio.2005.09.001

Diagnostic Microbiology & Infectious Disease
Volume 54, Issue 1 , Pages 7-12 , January 2006

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