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Characterization of ST80 Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus clone in Tunisia

  • Mouna Ben Nejma

      Affiliations

    • High Institute of Biotechnology, Medical Biotechnology, Monastir, UR-09-11, Tunisia 5000, Tunisia
    • ,
  • Maha Mastouri

      Affiliations

    • Laboratory of Microbiology, CHU, F. Bourguiba, Monastir, Tunisia 5000, Tunisia
    • ,
  • Besma Bel Hadj Jrad

      Affiliations

    • High Institute of Biotechnology, Medical Biotechnology, Monastir, UR-09-11, Tunisia 5000, Tunisia
    • ,
  • Mohamed Nour

      Affiliations

    • High Institute of Biotechnology, Medical Biotechnology, Monastir, UR-09-11, Tunisia 5000, Tunisia
    • Corresponding Author InformationCorresponding author. Tel.: +216-98547828; fax: +216-73465404.

Received 5 December 2007; accepted 15 February 2008. published online 04 April 2008.
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Abstract 

The spread of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has been reported in communities worldwide. In this study, we characterized 64 Tunisian CA-MRSA by agr typing, polymerase chain reaction assay for 20 virulence genes, staphylococcal chromosomal cassette mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and protein A gene (spa) typing. All our isolates were lukS-PV-lukF-PV positive, etd positive, and edin positive. They harbored SCCmec type IV and belonged to agr group 3. PFGE typing showed that our isolates were distributed in 11 different pulsotypes. spa typing and MLST, performed with isolates representative of each PFGE pattern, revealed that all isolates had a unique spa type (t044) and a common sequence type (ST80). The isolates showed susceptibility to the majority of antibiotics, and resistance to kanamycin, erythromycin, and tetracycline, but intermediate resistance to fusidic acid. Full analysis of our results revealed that our isolates were nonmultiresistant and belonged to a single clonal type ST80.

Keywords: MRSA, PVL, PFGE, ST80, Spa typing

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PII: S0732-8893(08)00146-6

doi:10.1016/j.diagmicrobio.2008.02.010

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