Use of SAG2A recombinant Toxoplasma gondii surface antigen as a diagnostic marker for human acute toxoplasmosis: analysis of titers and avidity of IgG and IgG1 antibodies

Béla, Samantha Ribeiro; Oliveira Silva, Deise A.; Cunha-Júnior, Jair Pereira; Pirovani, Carlos P.; Chaves-Borges, Flávia Andrade; Reis de Carvalho, Fernando; Carrijo de Oliveira, Taísa; Mineo, José Roberto

doi:10.1016/j.diagmicrobio.2008.05.017

« Previous Next »Diagnostic Microbiology & Infectious Disease
Volume 62, Issue 3 , Pages 245-254, November 2008

Use of SAG2A recombinant Toxoplasma gondii surface antigen as a diagnostic marker for human acute toxoplasmosis: analysis of titers and avidity of IgG and IgG1 antibodies

Samantha Ribeiro Béla
- Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, 38401-136, Brazil
Deise A. Oliveira Silva
- Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, 38401-136, Brazil
Jair Pereira Cunha-Júnior
- Division of Immunology, Federal University of Tocantins, Palmas, TO, 77020-210, Brazil
Carlos P. Pirovani
- Division of Microbiology and Immunology, Department of Biological Sciences, State University of Santa Cruz, Ilhéus, BA, 45662-000, Brazil
Flávia Andrade Chaves-Borges
- Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, 38401-136, Brazil
Fernando Reis de Carvalho
- Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, 38401-136, Brazil
Taísa Carrijo de Oliveira
- Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, 38401-136, Brazil
José Roberto Mineo
- Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, 38401-136, Brazil
- Corresponding author. Tel.: +55-34-3218-2195; fax: +55-34-3218-2333.

Received 11 January 2008; accepted 26 May 2008. published online 14 August 2008.

Abstract

We evaluated the reactivity of IgG and IgG1 antibodies by immunoassays in sera from patients with acute and chronic phases of toxoplasmosis against 2 recombinant antigens, SAG2A (full molecule) and SAG2AΔ (truncated molecule from the epitope recognized by A4D12 monoclonal antibody [mAb]), in comparison with soluble Toxoplasma antigen (STAg). Results demonstrated higher IgG reactivity in acute sera with both STAg and SAG2A than in chronic phase sera, and this difference was more evident for IgG1 antibodies to SAG2A. Low reactivity to SAG2AΔ was found in sera from both phases. ELISA-IgG-SAG2A showed high sensitivity (95%) and specificity (100%). ELISA-IgG1-SAG2A sensitivity was significantly higher (90%) for acute than for chronic (67%) phases. ELISA-IgG avidity using STAg demonstrated high performance for characterizing sera with high avidity (>60%), whereas the ELISA-IgG1 avidity-SAG2A immunoassay was the best to define chronic phase infection. It can be concluded that SAG2A is an antigen that may be used as a diagnostic tool to characterize the acute phase Toxoplasma gondii infection. Also, the epitope recognized by A4D12 mAb may be critical for the recognition of this molecule.

Keywords: Toxoplasma gondii, Human toxoplasmosis, Antibody avidity, IgG subclasses, SAG2A molecule, Recombinant antigen, Serologic diagnosis