Diagnostic Microbiology & Infectious Disease
Volume 62, Issue 3 , Pages 272-279 , November 2008

A duplex real-time reverse transcriptase polymerase chain reaction assay for the detection of St. Louis encephalitis and eastern equine encephalitis viruses

  • Rene Hull

      Affiliations

    • Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
  • ,
  • Seela Nattanmai

      Affiliations

    • Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
    • Present address: Albany Medical Center, Albany, NY.
  • ,
  • Laura D. Kramer

      Affiliations

    • Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
    • Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY 12222, USA
  • ,
  • Kristen A. Bernard

      Affiliations

    • Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
    • Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY 12222, USA
  • ,
  • Norma P. Tavakoli

      Affiliations

    • Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
    • Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY 12222, USA
    • Corresponding Author InformationCorresponding author. Empire State Plaza, PO Box 509, Albany, NY 12201. Tel.: +1-518-474-3046; fax: +1-518-486-2693.

Received 18 April 2008 ,Accepted 9 July 2008.

References 

  1. Brault AC, Powers AM, Chavez CLV, Lopez RN, Cachon MF, Gutierrez LFL, et al. Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central and South America. Am. J. Trop. Med. Hyg. 1999;61:579–586
  2. Briese T, Glass WG, Lipkin WI. Detection of West Nile virus sequences in cerebrospinal fluid. Lancet. 2000;355:1614–1615
  3. Bronzoni RV, Moreli ML, Cruz ACR, Figueiredo LT. Multiplex nested PCR for Brazilian Alphavirus diagnosis. Trans. R. Soc. Trop. Med. Hyg. 2004;98:456–461
  4. Bronzoni RV, Baleotti FG, Nogueira RM, Nunes M, Figueiredo LT. Duplex reverse transcription-PCR followed by nested PCR assays for detection and identification of Brazilian alphaviruses and flaviviruses. J. Clin. Microbiol. 2005;43:696–702
  5. Calisher CH. Medically important arboviruses of the United States and Canada. Clin. Microbiol. Rev. 1994;7:89–116
  6. Cirino N, Tavakoli NP, Madison-Antenucci S, Egan C. Real-time multiplex PCR. In:  McKay IM editors. Real-Time PCR in Microbiology: From Diagnosis to Characterization. Norfolk, UK: Horizon Scientific Press; 2007;p. 183–229.
  7. Dalrymple JM, Schlesinger S, Russell PK. Antigenic characterization of two Sindbis envelope glycoproteins separated by isoelectric focusing. Virology. 1976;69:93–103
  8. Deresiewicz RL, Thaler SJ, Hsu L, Zamani AA. Clinical and neuroradiographic manifestations of eastern equine encephalitis. N. Engl. J. Med. 1997;336:1867–1874
  9. Fulop L, Barret AD, Phillpotts R, Martin K, Leslie D, Titbal R. Rapid identification of flaviviruses based on conserved NS5 gene sequences. J. Virol. Methods. 1993;44:179–188
  10. Iacono-Connors LC, Schmaljohn CS. Cloning and sequence analysis of the genes encoding the non-structural proteins of Langat virus and comparative analysis with other flaviviruses. Virology. 1992;188:875–880
  11. Lambert AJ, Martin DA, Lanciotti RS. Detection of North American Eastern and Western equine encephalitis viruses by nucleic acid amplification assays. J. Clin. Microbiol. 2003;41:379–385
  12. Lanciotti RS, Kerst AJ. Nucleic acid sequence-based amplification assays for rapid detection of West Nile virus and St. Louis encephalitis viruses. J. Clin. Microbiol. 2001;39:4506–4513
  13. Lee J-H, Tennessen K, Lilley BG, Unnasch TR. Simultaneous detection of three mosquito-borne encephalitis viruses (Eastern equine, La Crosse, and St. Louis) with single-tube multiplex reverse transcriptase polymerase chain reaction assay. J. Am. Mosq. Control Assoc. 2002;18:26–31
  14. Linssen B, Kinney RM, Aguilar P, Russell KL, Watts DM, Kaaden OR, et al. Development of reverse transcription-PCR assays specific for detection of equine encephalitis viruses. J. Clin. Microbiol. 2000;38:1527–1535
  15. Martin DA, Noga A, Kosoy O, Johnson AJ, Petersen LR, Lanciotti RS. Evaluation of a diagnostic algorithm using immunoglobulin M enzyme-linked immunosorbent assay to differentiate human West Nile virus and St. Louis encephalitis virus infections during the 2002 West Nile virus epidemic in the United States. Clin. Diagn. Lab. Immunol. 2004;11:1130–1133
  16. O'Guinn ML, Lee JS, Kondig JP, Fernandez R, Carbajal F. Field detection of Eastern equine encephalitis virus in the Amazon Basin region of Peru using reverse transcription-polymerase chain reaction adapted for field identification of arthropod-borne pathogens. Am. J. Trop. Med. Hyg. 2004;70:164–171
  17. Pfeffer M, Proebster B, Kinney RM, Kaaden OR. Genus-specific detection of alphaviruses by a semi-nested reverse transcription-polymerase chain reaction. Am. J. Trop. Med. Hyg. 1997;57:709–718
  18. Powers AM, Brault AC, Shirako Y, Strauss EG, Kang W, Strauss JH, et al. Evolutionary relationships and systematics of the alphaviruses. J. Virol. 2001;75:10118–10131
  19. Roehrig GT. The use of monoclonal antibodies in studies of the structural proteins of togaviruses and flaviviruses. In:  Schlesinger MJ,  Schlesinger S editor. The Togaviridae and Flaviviridae. New York: Plenum Press; 1986;p. 251–278
  20. Russell RC, Dwyer DE. Arboviruses associated with human disease in Australia. Microb. Infect. 2000;2:1693–1704
  21. Sanchez-Seco MP, Rosario D, Quiroz E, Guzman G, Tenorio A. A generic nested-RT-PCR followed by sequencing for detection and identification of members of the alphavirus genus. J. Virol. Methods. 2001;95:153–161
  22. Scaramozzino N, Crance JM, Jouan A, DeBriel DA, Stoll F, Garin D. Comparison of flavivirus primer pairs and development of a rapid, highly sensitive reverse transcription-PCR assay for detection of flavivirus targeted to a conserved region of the NS5 gene sequences. J. Clin. Microbiol. 2001;39:1922–1927
  23. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007;24:1596–1599
  24. Tavakoli NP, Nattanmai S, Hull R, Fusco H, Dzigua L, Wang H, et al. Detection and typing of human herpes virus 6 by molecular methods in specimens from patients diagnosed with encephalitis/meningitis. J. Clin. Microbiol. 2007;45:3972–3978
  25. Tsai TF, Mitchell CJ. St. Louis encephalitis. In:  Monath TP editors. The Arboviruses: Epidemiology and Ecology. Vol. 4:Boca Raton, FL: CRC Press; 1989;p. 113–143
  26. Wells DL, Buff E, Lewis AL, Calder RA. Epidemiologic notes and reports update: arboviral surveillance—Florida, 1990. Morb. Mortal. Wkly. Rep. 1990;39:650–651
  27. Wolcott JA, Wust CJ, Brown A. Identification of immunologically cross-reactive proteins of Sindbis virus: evidence for unique conformation of E1 glycoprotein from infected cells. J. Virol. 1984;49:379–385

PII: S0732-8893(08)00312-X

doi: 10.1016/j.diagmicrobio.2008.07.004

Diagnostic Microbiology & Infectious Disease
Volume 62, Issue 3 , Pages 272-279 , November 2008