Diagnostic Microbiology & Infectious Disease
Volume 62, Issue 4 , Pages 464-466 , December 2008

Percent positive rate of Lyme real-time polymerase chain reaction in blood, cerebrospinal fluid, synovial fluid, and tissue

  • Ngolela Esther Babady

      Affiliations

    • Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    • ,
  • Lynne M. Sloan

      Affiliations

    • Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    • ,
  • Emily A. Vetter

      Affiliations

    • Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    • ,
  • Robin Patel

      Affiliations

    • Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    • Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    • ,
  • Matthew J. Binnicker

      Affiliations

    • Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-507-538-1640; fax: +1-507-266-4341.

Received 12 June 2008 ,Accepted 19 August 2008.

References 

  1. Aguero-Rosenfeld ME, Wang G, Schwartz I, Wormser GP. Diagnosis of Lyme borreliosis. Clin. Microbiol. Rev. 2005;18:484–509
  2. Brettschneider S, Bruckbauer H, Klugbauer N, Hofmann H. Diagnostic value of PCR for detection of Borrelia burgdorferi in skin biopsy and urine samples from patients with skin borreliosis. J. Clin. Microbiol. 1998;36:2658–2665
  3. Gooskens J, Templeton KE, Claas EC, van Dam AP. Evaluation of an internally controlled real-time PCR targeting the ospA gene for detection of Borrelia burgdorferi sensu lato DNA in cerebrospinal fluid. Clin. Microbiol. Infect. 2006;12:894–900
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  6. Huppertz HI, Schmidt H, Karch H. Detection of Borrelia burgdorferi by nested polymerase chain reaction in cerebrospinal fluid and urine of children with neuroborreliosis. Eur. J. Pediatr. 1993;152:414–417
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  9. Portnoi D, Sertour N, Ferquel E, Garnier M, Baranton G, Postic D. A single-run, real-time PCR for detection and identification of Borrelia burgdorferi sensu lato species, based on the hbb gene sequence. FEMS Microbiol. Lett. 2006;259:35–40
  10. Rauter C, Mueller M, Diterich I, Zeller S, Hassler D, Meergans T, et al. Critical evaluation of urine-based PCR assay for diagnosis of Lyme borreliosis. Clin. Diagn. Lab. Immunol. 2005;12:910–917
  11. Reed KD. Laboratory testing for Lyme disease: possibilities and practicalities. J. Clin. Microbiol. 2002;40:319–324
  12. Schwaiger M, Peter O, Cassinotti P. Routine diagnosis of Borrelia burgdorferi (sensu lato) infections using a real-time PCR assay. Clin. Microbiol. Infect. 2001;7:461–469
  13. Tang YW, Hibbs JR, Tau KR, Qian Q, Skarhus HA, Smith TF, et al. Effective use of polymerase chain reaction for diagnosis of central nervous system infections. Clin. Infect. Dis. 1999;29:803–806

 The present study was presented in part at the 108th General Meeting of the American Society for Microbiology in Boston, MA, June 1-5, 2008 (Abstract C-052).

PII: S0732-8893(08)00377-5

doi: 10.1016/j.diagmicrobio.2008.08.016

Diagnostic Microbiology & Infectious Disease
Volume 62, Issue 4 , Pages 464-466 , December 2008

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