Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 204-206, February 2010

Direct detection of Streptococcus pneumoniae in positive blood cultures by real-time polymerase chain reaction

  • Laura Selva

      Affiliations

    • Molecular Microbiology Department, University Hospital Sant Joan de Déu, 08950 Esplugues-Barcelona, Spain
    • ,
  • Cristina Esteva

      Affiliations

    • Molecular Microbiology Department, University Hospital Sant Joan de Déu, 08950 Esplugues-Barcelona, Spain
    • ,
  • Amadeu Gené

      Affiliations

    • Molecular Microbiology Department, University Hospital Sant Joan de Déu, 08950 Esplugues-Barcelona, Spain
    • ,
  • Maria Fernández de Sevilla

      Affiliations

    • Paediatrics Department, University Hospital Sant Joan de Déu, Esplugues-Barcelona, Spain
    • ,
  • Susanna Hernandez-Bou

      Affiliations

    • Paediatrics Department, University Hospital Sant Joan de Déu, Esplugues-Barcelona, Spain
    • ,
  • Carmen Muñoz-Almagro

      Affiliations

    • Molecular Microbiology Department, University Hospital Sant Joan de Déu, 08950 Esplugues-Barcelona, Spain
    • Corresponding Author InformationCorresponding author. Tel.: +34-93-280-5569; fax: +34-93-280-3626.

Received 19 December 2008; accepted 11 May 2009.

Abstract 

We developed a real-time polymerase chain reaction specific for Streptococcus pneumoniae to be applied directly from blood culture bottles without previous DNA extraction step. For the 128 blood culture bottles tested, the assay had 94% and 98.4% sensitivity and specificity, respectively. This assay provides rapid and accurate identification of this pathogen.

Keywords: Real-time PCR, Blood culture bottles, Streptococcus pneumoniae

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PII: S0732-8893(09)00235-1

doi:10.1016/j.diagmicrobio.2009.05.023

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 204-206, February 2010

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