Diagnostic Microbiology & Infectious Disease
Volume 65, Issue 4 , Pages 435-438, December 2009

Comparison of nucleic acid extraction methods for the detection of Mycoplasma pneumoniae

Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA

Received 12 May 2009; accepted 8 August 2009. published online 22 September 2009.

Abstract 

Four nucleic acid extraction procedures (2 automated and 2 manual) were compared for their efficiency at isolating Mycoplasma pneumoniae DNA. Oropharyngeal swabs from healthy volunteers were spiked with varying amounts of M. pneumoniae, extracted, and tested using real-time polymerase chain reaction. Our data indicate that both automated extraction methods consistently outperform the manual procedures.

Keywords: Mycoplasma pneumoniae, Nucleic acid extraction, Community-acquired pneumonia

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PII: S0732-8893(09)00329-0

doi:10.1016/j.diagmicrobio.2009.08.001

Diagnostic Microbiology & Infectious Disease
Volume 65, Issue 4 , Pages 435-438, December 2009

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