Diagnostic Microbiology & Infectious Disease
Volume 65, Issue 4 , Pages 384-391 , December 2009

A rapid, 2-well, multiplex real-time polymerase chain reaction assay for the detection of SCCmec types I to V in methicillin-resistant Staphylococcus aureus

  • Håvard Valvatne

      Affiliations

    • Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, The Netherlands
    • ,
  • Michelle I.A. Rijnders

      Affiliations

    • Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, The Netherlands
    • ,
  • Ana Budimir

      Affiliations

    • Department of Clinical and Molecular Microbiology, Clinical Hospital Centre Zagreb, Croatia
    • ,
  • Marie-Louise Boumans

      Affiliations

    • Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, The Netherlands
    • ,
  • Albert J. de Neeling

      Affiliations

    • National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands
    • ,
  • Patrick S. Beisser

      Affiliations

    • Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, The Netherlands
    • ,
  • Ellen E. Stobberingh

      Affiliations

    • Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, The Netherlands
    • ,
  • Ruud H. Deurenberg

      Affiliations

    • Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, The Netherlands
    • Corresponding Author InformationCorresponding author. Tel.: +31-43-3874644; fax: +31-43-3876643.

Received 10 April 2009 ,Accepted 11 August 2009.

References 

  1. Berglund C, Ito T, Ikeda M, Ma XX, Soderquist B, Hiramatsu K. Novel type of staphylococcal cassette chromosome mec in a methicillin-resistant Staphylococcus aureus strain isolated in Sweden. Antimicrob. Agents Chemother. 2008;52:3512–3516
  2. Boye K, Bartels MD, Andersen IS, Moller JA, Westh H. A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V. Clin. Microbiol. Infect. 2007;13:725–727
  3. Chongtrakool P, Ito T, Ma XX, Kondo Y, Trakulsomboon S, Tiensasitorn C, et al. Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements. Antimicrob. Agents Chemother. 2006;50:1001–1012
  4. Daum RS, Ito T, Hiramatsu K, Hussain F, Mongkolrattanothai K, Jamklang M, et al. A novel methicillin-resistance cassette in community-acquired methicillin-resistant Staphylococcus aureus isolates of diverse genetic backgrounds. J. Infect. Dis. 2002;186:1344–1347
  5. Deurenberg RH, Vink C, Oudhuis GJ, Mooij JE, Driessen C, Coppens G, et al. Different clonal complexes of methicillin-resistant Staphylococcus aureus are disseminated in the Euregio Meuse-Rhine region. Antimicrob. Agents Chemother. 2005;49:4263–4271
  6. Donker GA, Deurenberg RH, Driessen C, Sebastian S, Nys S, Stobberingh EE. The population structure of Staphylococcus aureus among general practice patients from the Netherlands. Clin. Microbiol. Infect. 2009;15:137–143
  7. Ender M, Burger S, Sokoli A, Zbinden R, Berger-Bachi B, Heusser R, et al. Variability of SCCmec in the Zurich area. Eur. J. Clin. Microbiol. Infect. Dis. 2009;28:647–653
  8. Friedrich AW, Witte W, Harmsen D, de Lencastre H, Hryniewicz W, Scheres J, et al. SeqNet.org: a European laboratory network for sequence-based typing of microbial pathogens. Euro Surveill. 2006;11:112–114
  9. Hallin M, Deplano A, Denis O, De Mendonca R, De Ryck R, Struelens MJ. Validation of pulsed-field gel electrophoresis and spa typing for long-term, nationwide epidemiological surveillance studies of Staphylococcus aureus infections. J. Clin. Microbiol. 2007;45:127–133
  10. Heusser R, Ender M, Berger-Bachi B, McCallum N. Mosaic staphylococcal cassette chromosome mec containing two recombinase loci and a new mec complex, B2. Antimicrob. Agents Chemother. 2007;51:390–393
  11. Ito T, Katayama Y, Hiramatsu K. Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin–resistant Staphylococcus aureus N315. Antimicrob. Agents Chemother. 1999;43:1449–1458
  12. Ito T, Katayama Y, Asada K, Mori N, Tsutsumimoto K, Tiensasitorn C, et al. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 2001;45:1323–1336
  13. Ito T, Ma XX, Takeuchi F, Okuma K, Yuzawa H, Hiramatsu K. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrob. Agents Chemother. 2004;48:2637–2651
  14. Kim J, Jeong JH, Cha HY, Jin JS, Lee JC, Lee YC, et al. Detection of diverse SCCmec variants in methicillin-resistant Staphylococcus aureus and comparison of SCCmec typing methods. Clin. Microbiol. Infect. 2007;13:1128–1130
  15. Klein D. Quantification using real-time PCR technology: applications and limitations. Trends Mol. Med. 2002;8:257–260
  16. Kondo Y, Ito T, Ma XX, Watanabe S, Kreiswirth BN, Etienne J, et al. Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob. Agents Chemother. 2007;51:264–274
  17. Kwon NH, Park KT, Moon JS, Jung WK, Kim SH, Kim JM, et al. Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. J. Antimicrob. Chemother. 2005;56:624–632
  18. Ma XX, Ito T, Tiensasitorn C, Jamklang M, Chongtrakool P, Boyle-Vavra S, et al. Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob. Agents Chemother. 2002;46:1147–1152
  19. Milheirico C, Oliveira DC, de Lencastre H. Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus: ‘SCCmec IV multiplex’. J. Antimicrob. Chemother. 2007;60:42–48
  20. Milheirico C, Oliveira DC, de Lencastre H. Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus. Antimicrob. Agents Chemother. 2007;51:3374–3377
  21. Nulens E, Stobberingh EE, van Dessel H, Sebastian S, van Tiel FH, Beisser PS, et al. Molecular characterization of Staphylococcus aureus bloodstream isolates collected in a Dutch university hospital between 1999 and 2006. J. Clin. Microbiol. 2008;46:2438–2441
  22. Okuma K, Iwakawa K, Turnidge JD, Grubb WB, Bell JM, O'Brien FG, et al. Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. J. Clin. Microbiol. 2002;40:4289–4294
  23. Oliveira DC, de Lencastre H. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 2002;46:2155–2161
  24. Oliveira DC, Milheirico C, de Lencastre H. Redefining a structural variant of staphylococcal cassette chromosome mec, SCCmec type VI. Antimicrob. Agents Chemother. 2006;50:3457–3459
  25. Qi W, Ender M, O'Brien F, Imhof A, Ruef C, McCallum N, et al. Molecular epidemiology of methicillin-resistant Staphylococcus aureus in Zurich, Switzerland (2003): prevalence of type IV SCCmec and a new SCCmec element associated with isolates from intravenous drug users. J. Clin. Microbiol. 2005;43:5164–5170
  26. Shore A, Rossney AS, Keane CT, Enright MC, Coleman DC. Seven novel variants of the staphylococcal chromosomal cassette mec in methicillin-resistant Staphylococcus aureus isolates from Ireland. Antimicrob. Agents Chemother. 2005;49:2070–2083
  27. Strommenger B, Kettlitz C, Weniger T, Harmsen D, Friedrich AW, Witte W. Assignment of Staphylococcus isolates to groups by spa typing, SmaI macrorestriction analysis, and multilocus sequence typing. J. Clin. Microbiol. 2006;44:2533–2540
  28. Strommenger B, Heuck D, Schmidt C, Pasemann B, Nubel U, Witte W. spa typing of Staphylococcus aureus as a frontline tool in epidemiological typing. J. Clin. Microbiol. 2008;46:574–581
  29. Takano T, Higuchi W, Otsuka T, Baranovich T, Enany S, Saito K, et al. Novel characteristics of community-acquired methicillin-resistant Staphylococcus aureus belonging to multilocus sequence type 59 in Taiwan. Antimicrob. Agents Chemother. 2008;52:837–845
  30. Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 2005;43:5026–5033
  31. Zhang K, McClure JA, Elsayed S, Conly JM. Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 2009;53:531–540

PII: S0732-8893(09)00335-6

doi: 10.1016/j.diagmicrobio.2009.08.006

Diagnostic Microbiology & Infectious Disease
Volume 65, Issue 4 , Pages 384-391 , December 2009

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