In vivo selection of carbapenem-resistant Klebsiella pneumoniae by OmpK36 loss during meropenem treatment

Received 11 June 2009; accepted 17 August 2009. published online 22 September 2009.

Abstract

We recovered a carbapenem-resistant Klebsiella pneumoniae isolate H224 under in vivo meropenem selection pressure. Insertional inactivation of a major porin gene, ompK36, by IS5 element might play a role in acquiring carbapenem resistance in this strain harboring plasmid-borne DHA-1 AmpC β-lactamase.

Keywords: OmpK35, OmpK36, DHA-1, Carbapenems, IS5

a Department of Laboratory Medicine, Hallym University College of Medicine, 120-950 Seoul, Republic of Korea

b Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 120-752 Seoul, Republic of Korea

c Department of Internal Medicine, Yonsei University College of Medicine, 120-752 Seoul, Republic of Korea

d Department of Internal Medicine, Hallym University College of Medicine, 120-950 Seoul, Republic of Korea

e Department of Laboratory Medicine, CHA University School of Medicine, 463-712 Sungnam, Republic of Korea

Corresponding author. Tel.: +82-2-2228-2448; fax: +82-2-313-0908.

PII: S0732-8893(09)00352-6

doi:10.1016/j.diagmicrobio.2009.08.010

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