Diagnostic Microbiology & Infectious Disease
Volume 65, Issue 4 , Pages 447-449 , December 2009

In vivo selection of carbapenem-resistant Klebsiella pneumoniae by OmpK36 loss during meropenem treatment

  • Wonkeun Song

      Affiliations

    • Department of Laboratory Medicine, Hallym University College of Medicine, 120-950 Seoul, Republic of Korea
    • ,
  • Borum Suh

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 120-752 Seoul, Republic of Korea
    • ,
  • Jun Yong Choi

      Affiliations

    • Department of Internal Medicine, Yonsei University College of Medicine, 120-752 Seoul, Republic of Korea
    • ,
  • Seok Hoon Jeong

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 120-752 Seoul, Republic of Korea
    • Corresponding Author InformationCorresponding author. Tel.: +82-2-2228-2448; fax: +82-2-313-0908.
    • ,
  • Eun Hee Jeon

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 120-752 Seoul, Republic of Korea
    • ,
  • Young Ki Lee

      Affiliations

    • Department of Internal Medicine, Hallym University College of Medicine, 120-950 Seoul, Republic of Korea
    • ,
  • Seong Geun Hong

      Affiliations

    • Department of Laboratory Medicine, CHA University School of Medicine, 463-712 Sungnam, Republic of Korea
    • ,
  • Kyungwon Lee

      Affiliations

    • Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 120-752 Seoul, Republic of Korea

Received 11 June 2009 ,Accepted 17 August 2009.

References 

  1. Doumith M, Ellington MJ, Livermore DM, Woodford N. Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK. J Antimicrob Chemother. 2009;63:659–667
  2. Elliott E, Brink AJ, van Greune J, Els Z, Woodford N, Turton J, et al. In vivo development of ertapenem resistance in a patient with pneumonia caused by Klebsiella pneumoniae with an extended-spectrum β-lactamase. Clin Infect Dis. 2006;42:e95–e98
  3. Kaczmarek FM, Dib-Hajj F, Shang W, Gootz TD. High-level carbapenem resistance in a Klebsiella pneumoniae clinical isolate is due to the combination of blaACT-1 β-lactamase production, porin OmpK35/36 insertional inactivation, and down-regulation of the phosphate transport porin PhoE. Antimicrob Agents Chemother. 2006;50:3396–3406
  4. Lee K, Chong Y, Shin HB, Kim YA, Yong D, Yum JH. Modified Hodge and EDTA-disk synergy tests to screen metallo-β-lactamase–producing strains of Pseudomonas and Acinetobacter species. Clin Microbiol Infect. 2001;7:88–91
  5. Lee CH, Chu C, Liu JW, Chen YS, Chiu CJ, Su LH. Collateral damage of flomoxef therapy: in vivo development of porin deficiency and acquisition of blaDHA-1 leading to ertapenem resistance in a clinical isolate of Klebsiella pneumoniae producing CTX-M-3 and SHV-5 β-lactamases. J Antimicrob Chemother. 2007;60:410–413
  6. Lee K, Yong D, Choi YS, Yum JH, Kim JM, Woodford N, et al. Reduced imipenem susceptibility in Klebsiella pneumoniae clinical isolates with plasmid-mediated CMY-2 and DHA-1 β-lactamases co-mediated by porin loss. Intern J Antimicrob Agents. 2007;29:201–206
  7. Nordmann P, Cuzon G, Naas T. The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. Lancet Infect Dis. 2009;9:228–236
  8. Queenan AM, Bush K. Carbapenemases: the versatile β-lactamases. Clin Microbiol Rev. 2007;20:440–458
  9. Sawers RG. Transcript analysis of Escherichia coli K-12 insertion element IS5. FEMS Microbiol Lett. 2005;244:397–401
  10. Song W, Bae IK, Lee YN, Lee CH, Lee SH, Jeong SH. Detection of extended-spectrum β-lactamases by using boronic acid as an AmpC β-lactamase inhibitor in clinical isolates of Klebsiella spp. and Escherichia coli. J Clin Microbiol. 2007;45:1180–1184
  11. Yigit H, Queenan AM, Anderson GJ, Domenech-Sanchez A, Biddle JW, Steward CD, et al. Novel carbapenem-hydrolyzing β-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother. 2001;45:1151–1161

PII: S0732-8893(09)00352-6

doi: 10.1016/j.diagmicrobio.2009.08.010

Diagnostic Microbiology & Infectious Disease
Volume 65, Issue 4 , Pages 447-449 , December 2009

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