Diagnostic Microbiology & Infectious Disease
Volume 65, Issue 4 , Pages 379-383 , December 2009

Characterization of plasmids encoding CMY-2 AmpC β-lactamases from Escherichia coli in Canadian intensive care units

  • Patricia J. Baudry

      Affiliations

    • Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada R3A 1R9
    • Department of Clinical Microbiology, Health Sciences Centre, Winnipeg, Canada R3A 1R9
    • Corresponding Author InformationCorresponding author. Department of Clinical Microbiology, Health Sciences Centre, Winnipeg, Manitoba, Canada R3A 1R9. Tel.: +1-204-787-4684; fax: +1-204-787-4699.
    • ,
  • Laura Mataseje

      Affiliations

    • Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada R3A 1R9
    • National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada R3E 3R2
    • ,
  • George G. Zhanel

      Affiliations

    • Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada R3A 1R9
    • Department of Clinical Microbiology, Health Sciences Centre, Winnipeg, Canada R3A 1R9
    • ,
  • Daryl J. Hoban

      Affiliations

    • Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada R3A 1R9
    • Department of Clinical Microbiology, Health Sciences Centre, Winnipeg, Canada R3A 1R9
    • ,
  • Michael R. Mulvey

      Affiliations

    • Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada R3A 1R9
    • National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada R3E 3R2

Received 14 May 2009 ,Accepted 14 August 2009.

References 

  1. Baudry PJ, Nichol K, DeCorby M, Mataseje L, Mulvey MR, Hoban DJ, et al. Comparison of antimicrobial resistance profiles among extended-spectrum-beta-lactamase–producing and acquired AmpC beta-lactamase–producing Escherichia coli isolates from Canadian intensive care units. Antimicrob. Agents Chemother. 2008;52:1846–1849
  2. Boyd DA, Tyler S, Christianson S, McGeer A, Muller MP, Willey BM, et al. Complete nucleotide sequence of a 92-kilobase plasmid harboring the CTX-M-15 extended-spectrum beta-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada. Antimicrob. Agents Chemother. 2004;48:3758–3764
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  10. Deshpande LM, Jones RN, Fritsche TR, Sader HS. Occurrence of plasmidic AmpC type beta-lactamase–mediated resistance in Escherichia coli: report from the SENTRY Antimicrobial Surveillance Program (North America, 2004). Int. J. Antimicrob. Agents. 2006;28:578–581
  11. Goyanes MJ, Cercenado E, Insa R, Morente A, Alcala L, Bouza E. High rates of antimicrobial co-resistance among Enterobacteriaceae: comparative analysis between clinical isolates resistant and susceptible to third-generation cephalosporins. Rev. Esp. Quimioter. 2007;20:216–221
  12. Hopkins KL, Liebana E, Villa L, Batchelor M, Threlfall EJ, Carattoli A. Replicon typing of plasmids carrying CTX-M or CMY beta-lactamases circulating among Salmonella and Escherichia coli isolates. Antimicrob. Agents Chemother. 2006;50:3203–3206
  13. Jacoby GA. AmpC beta-lactamases. Clin. Microbiol. Rev. 2009;22:161–182
  14. Mataseje LF, Neumann N, Crago B, Baudry P, Zhanel GG, Louie M, et al. Characterization of cefoxitin-resistant Escherichia coli isolated from recreational beaches and private drinking water in Canada between 2004 and 2006. Antimicrob. Agents Chemother. 2009;53:3126–3130
  15. Mulvey MR, Bryce E, Boyd D, Ofner-Agostini M, Christianson S, Simor AE, et al. Ambler class A extended-spectrum beta-lactamase–producing Escherichia coli and Klebsiella spp. in Canadian hospitals. Antimicrob. Agents Chemother. 2004;48:1204–1214
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  18. Perez-Perez FJ, Hanson ND. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J. Clin. Microbiol. 2002;40:2153–2162
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  22. Zhanel GG, DeCorby M, Laing N, Weshnoweski B, Vashisht R, Tailor F, et al Antimicrobial-resistant pathogens in intensive care units in Canada: results of the Canadian National Intensive Care Unit (CAN-ICU) study, 2005–2006. Antimicrob. Agents Chemother. 2008;52:1430–1437

☆ This article was presented in part at the American Society for Microbiology 108th General Meeting (2008) in Boston, MA.

☆☆ Patricia Baudry is supported by the Winnipeg Health Sciences Centre Department of Research, the Winnipeg Health Sciences Centre Foundation, and the Manitoba Health Research Council. This research is funded in part by Abott, Bayer Canada, Bristol-Myers Squibb, Janssen-Ortho, Merck Frosst, Sanofi-Aventis, and Wyeth.

PII: S0732-8893(09)00353-8

doi: 10.1016/j.diagmicrobio.2009.08.011

Diagnostic Microbiology & Infectious Disease
Volume 65, Issue 4 , Pages 379-383 , December 2009

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