Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 210-213 , February 2010

CTX-M and ampC β-lactamases contributing to increased prevalence of ceftriaxone-resistant Escherichia coli in Changi General Hospital, Singapore

  • Thean Yen Tan

      Affiliations

    • Department of Laboratory Medicine, Changi General Hospital, Singapore 529889, Singapore
    • Corresponding Author InformationCorresponding author. Tel.: +65-68504934; fax: +65-64269507.
    • ,
  • Lily Siew Yong Ng

      Affiliations

    • Department of Laboratory Medicine, Changi General Hospital, Singapore 529889, Singapore
    • ,
  • Jie He

      Affiliations

    • Department of Pathology, Singapore General Hospital, Singapore, 169608
    • ,
  • Li Yang Hsu

      Affiliations

    • Department of Medicine, National University of Singapore, Singapore, 119074

Received 5 May 2009 ,Accepted 30 August 2009.

References 

  1. Ben-Ami R, Schwaber MJ, Navon-Venezia S, Schwartz D, Giladi M, Chmelnitsky I, et al. Influx of extended-spectrum beta-lactamase–producing Enterobacteriaceae into the hospital. Clin. Infect. Dis. 2006;42:925–934
  2. Clinical Laboratory Standards Institute . Performance standards for antimicrobial susceptibility testing: 17th information supplement (M100-S17). Wayne, PA: CLSI; 2007;
  3. Jonas D, Spitzmuller B, Weist K, Ruden H, Daschner FD. Comparison of PCR-based methods for typing Escherichia coli. Clin. Microbiol. Infect. 2003;9:823–831
  4. Livermore DM, Woodford N. The beta-lactamase threat in Enterobacteriaceae, Pseudomonas and Acinetobacter. Trends Microbiol. 2006;14:413–420
  5. Monstein HJ, Ostholm-Balkhed A, Nilsson MV, Nilsson M, Dornbusch K, Nilsson LE. Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae. APMIS. 2007;115:1400–1408
  6. Perez-Perez FJ, Hanson ND. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J. Clin. Microbiol. 2002;40:2153–2162
  7. Pitout JD, Gregson DB, Church DL, Laupland KB. Population-based laboratory surveillance for AmpC beta-lactamase–producing Escherichia coli, Calgary. Emerg. Infect. Dis. 2007;13:443–448
  8. Tan TY, Ng SY, Teo L, Koh Y, Teok CH. Detection of plasmid-mediated AmpC in Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis. J. Clin. Pathol. 2008;61:642–644
  9. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 1995;33:2233–2239
  10. Woodford N, Fagan EJ, Ellington MJ. Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum (beta)-lactamases. J. Antimicrob. Chemother. 2006;57:154–155

☆ Tan Thean Yen, TYT, has received research funding from Wyeth Pharmaceuticals and has been an invited speaker for Wyeth Pharmaceuticals, Astra-Zeneca, and Jansson-Cilag. Hsu Li Yang, HLY, has received research funding from Wyeth Pharmaceuticals.

☆☆ Preliminary data from this study was presented as a poster at the Australian Society of Microbiology, Melbourne, July 6 to 10, 2008.

PII: S0732-8893(09)00363-0

doi: 10.1016/j.diagmicrobio.2009.08.019

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 210-213 , February 2010

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