Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 135-139 , February 2010

A rapid, simple, and sensitive loop-mediated isothermal amplification method to detect toxigenic Vibrio cholerae in rectal swab samples

  • Kazuhisa Okada

      Affiliations

    • Section of Bacterial Infections, Thailand–Japan Research Collaboration Center on Emerging and Reemerging Infections (RCC-ERI), DMSc, Ministry of Public Health, Nonthaburi 11000, Thailand
    • Corresponding Author InformationCorresponding author. DMSc, Thailand-Japan RCC-ERI, Ministry of Public Health, Nonthaburi 11000, Thailand. Tel.: +66-2-965-9748; fax: +66-2-965-9749.
    • ,
  • Siriporn Chantaroj

      Affiliations

    • National Institute of Health, DMSc, Tiwanond Road, Nonthaburi 11000, Thailand
    • ,
  • Tooru Taniguchi

      Affiliations

    • Section of Bacterial Infections, Thailand–Japan Research Collaboration Center on Emerging and Reemerging Infections (RCC-ERI), DMSc, Ministry of Public Health, Nonthaburi 11000, Thailand
    • ,
  • Yasuhiko Suzuki

      Affiliations

    • Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, N20, W10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan
    • ,
  • Amonrattana Roobthaisong

      Affiliations

    • Section of Bacterial Infections, Thailand–Japan Research Collaboration Center on Emerging and Reemerging Infections (RCC-ERI), DMSc, Ministry of Public Health, Nonthaburi 11000, Thailand
    • ,
  • Orapim Puiprom

      Affiliations

    • Section of Bacterial Infections, Thailand–Japan Research Collaboration Center on Emerging and Reemerging Infections (RCC-ERI), DMSc, Ministry of Public Health, Nonthaburi 11000, Thailand
    • ,
  • Takeshi Honda

      Affiliations

    • Section of Bacterial Infections, Thailand–Japan Research Collaboration Center on Emerging and Reemerging Infections (RCC-ERI), DMSc, Ministry of Public Health, Nonthaburi 11000, Thailand
    • ,
  • Pathom Sawanpanyalert

      Affiliations

    • National Institute of Health, DMSc, Tiwanond Road, Nonthaburi 11000, Thailand

Received 17 April 2009 ,Accepted 2 September 2009.

References 

  1. AI-Soud WA, Radstrom P. Purification and characterization of PCR-inhibitory components in blood cells. J. Clin. Microbiol. 2001;39:485–493
  2. Faruque SM, Asadulghani AR, Albert MJ, Islam KM, Mekalanos JJ. Induction of the lysogenic phage encoding cholera toxin in naturally occurring strains of toxigenic Vibrio cholerae O1 and O139. Infect. Immun. 1998;66:3752–3757
  3. Hara-Kudo Y, Yoshino M, Kojima T, Ikedo M. Loop-mediated isothermal amplification for the rapid detection of Salmonella. FEMS Microbiol. Lett. 2005;253:155–161
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  9. Kobayashi K, Seto K, Akasaka S, Makino M. Detection of toxigenic Vibrio cholerae O1 using polymerase chain reaction for amplifying the cholera enterotoxin gene. Kansenshogaku Zasshi. 1990;64:1323–1329
  10. Miyagi K, Sano K, Morita C, Imura S, Morimatsu S, Goto T, et al. An improved method for detecting faecal Vibrio cholerae by PCR of the toxin A gene. J. Med. Microbiol. 1999;48:883–889
  11. Mori Y, Nagamine K, Tomita N, Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 2001;289:150–154
  12. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, et al. Loop-mediated isothermal amplification of DNA. Nucleic. Acids Res. 2000;28:E63
  13. Satcher D. Emerging infection: getting ahead of the cure. Emerg. Infect. Dis. 1995;1:1–6
  14. Tomita N, Mori Y, Kodama H, Notomi T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat. Protoc. 2008;3:877–882
  15. Wilson K. Preparation of genomic DNA from bacteria. In:  Ausubel FM,  Brent R,  Kingston RE,  Moore DD,  Seidman JG,  Smith JA,  Struhl K editor. Current protocols in molecular biology. New York: Wiley,; 1997;p. 2.4.1\–2.4.5
  16. World Health Organization . Isolation of Vibrio cholerae O1 in a peripheral laboratory. In: Guidelines for cholera control. Geneva: World Health Organization; 1993;p. 54–61
  17. Yamazaki W, Seto K, Taguchi M, Ishibashi M, Inoue K. Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loop-mediated isothermal amplification. BMC. Microbiol. 2008;8:94–100

PII: S0732-8893(09)00367-8

doi: 10.1016/j.diagmicrobio.2009.09.004

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 135-139 , February 2010

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