Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 217-221 , February 2010

Occurrence and genotyping using automated repetitive-sequence–based PCR of methicillin-resistant Staphylococcus aureus ST398 in Southeast Austria

  • Andrea J. Grisold

      Affiliations

    • Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A-8010 Graz, Austria
    • Corresponding Author InformationCorresponding author. Tel.: +43-316-380-4383; fax: +43-316-380-9650.
    • ,
  • Gernot Zarfel

      Affiliations

    • Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A-8010 Graz, Austria
    • ,
  • Martin Hoenigl

      Affiliations

    • Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A-8010 Graz, Austria
    • ,
  • Karina Krziwanek

      Affiliations

    • Department of Hygiene, Microbiology and Tropical Medicine, National Reference Centre for Nosocomial Infections and Antibiotic Resistance, Elisabethinen Hospital, A-4010 Linz, Austria
    • ,
  • Gebhard Feierl

      Affiliations

    • Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A-8010 Graz, Austria
    • ,
  • Lilian Masoud

      Affiliations

    • Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A-8010 Graz, Austria
    • ,
  • Eva Leitner

      Affiliations

    • Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A-8010 Graz, Austria
    • ,
  • Ute Wagner-Eibel

      Affiliations

    • Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A-8010 Graz, Austria
    • ,
  • Alexandra Badura

      Affiliations

    • Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A-8010 Graz, Austria
    • ,
  • Egon Marth

      Affiliations

    • Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A-8010 Graz, Austria

Received 30 May 2009 ,Accepted 2 September 2009.

References 

  1. Armand-Lefevre L, Ruimy R, Andremont A. Clonal comparison of Staphylococcus aureus isolates from healthy pig farmers, human controls, and pigs. Emerg. Infect. Dis. 2005;11:711–714
  2. Bens CC, Voss A, Klaassen CH. Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis. J. Clin. Microbiol. 2006;44:1875–1876
  3. Boye K, Bartels MD, Andersen IS, Møller JA, Westh H. A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V. Clin. Microbiol. Infect. 2007;13:725–727
  4. Clinical and Laboratory Standards Institute (CLSI) . Performance standards for antimicrobial disk susceptibility tests—10th edition, M02-A10. Wayne, PA: CLSI; 2009;
  5. Cuny C, Strommenger B, Witte W, Stanek C. Clusters of infections in horses with MRSA ST1, ST254, and ST398 in a veterinary hospital. Microb. Drug Resist. 2008;14:307–311
  6. Deplano A, Schuermans A, Van Eldere J, Witte W, Meugnier H, Etienne J, et al. Multicenter evaluation of epidemiological typing of methicillin-resistant Staphylococcus aureus strains by repetitive-element PCR analysis. J. Clin. Microbiol. 2000;38:3527–3533
  7. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 2000;38:1008–1015
  8. Healy M, Huong J, Bittner T, Lising M, Frye S, Raza S, et al. Microbial DNA typing by automated repetitive-sequence–based PCR. J. Clin. Microbiol. 2005;43:199–207
  9. Horan TC, Andrus M, Dudeck MA. CDC/NHSN surveillance definition of health care-associated infection and criteria for specific types of infections in the acute care setting. Am. J. Infect. Control. 2008;36:309–332
  10. Huijsdens XW, van Dijke BJ, Spalburg E, van Santen-Verheuvel MG, Heck ME, Pluister GN, et al. Community-acquired MRSA and pig-farming. Ann. Clin. Microbiol. Antimicrob. 2006;5:26
  11. Krziwanek K, Metz-Gercek S, Mittermayer H. Methicillin-resistant Staphylococcus aureus ST398 from human patients, upper Austria. Emerg. Infect. Dis. 2009;15:766–769
  12. Lina G, Piémont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, et al. Involvement of Panton–Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin. Infect. Dis. 1999;29:1128–1132
  13. Moodley A, Stegger M, Bagcigil AF, Baptiste KE, Loeffler A, Lloyd DH, et al. Spa typing of methicillin-resistant Staphylococcus aureus isolated from domestic animals and veterinary staff in the UK and Ireland. J. Antimicrob. Chemother. 2006;58:1118–1123
  14. Murchan S, Kaufmann ME, Deplano A, de Ryck R, Struelens M, Zinn CE, et al. Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J. Clin. Microbiol. 2003;41:1574–1585
  15. Ross TL, Merz WG, Farkosh M, Carroll KC. Comparison of an automated repetitive sequence-based PCR microbial typing system to pulsed-field gel electrophoresis for analysis of outbreaks of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 2005;43:5642–5647
  16. Ruppitsch W, Indra A, Stöger A, Mayer B, Stadlbauer S, Wewalka G, et al. Classifying spa types in complexes improves interpretation of typing results for methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 2006;44:2442–2448
  17. Shutt CK, Pounder JI, Page SR, Schaecher BJ, Woods GL. Clinical evaluation of the DiversiLab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains. J. Clin. Microbiol. 2005;43:1187–1192
  18. Springer B, Orendi U, Much P, Höger G, Ruppitsch W, Krziwanek K, et al. Methicillin-resistant Staphylococcus aureus: a new zoonotic agent?. Wien. Klin. Wochenschr. 2009;121:86–90
  19. van Belkum A, Melles DC, Peeters JK, van Leeuwen WB, van Duijkeren E, Huijsdens XW, et al. Methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 in pigs and humans. Emerg. Infect. Dis. 2008;14:479–483
  20. van Loo I, Huijsdens X, Tiemersma E, de Neeling A, van de Sande-Bruinsma N, Beaujean D, et al. Emergence of methicillin-resistant Staphylococcus aureus of animal origin in humans. Emerg. Infect. Dis. 2007;13:1834–1839
  21. Voss A, Loeffen F, Bakker J, Klaassen C, Wulf M. Methicillin-resistant Staphylococcus aureus in pig farming. Emerg. Infect. Dis. 2005;11:1965–1966
  22. Witte W, Strommenger B, Stanek C, Cuny C. Methicillin-resistant Staphylococcus aureus ST398 in humans and animals, Central Europe. Emerg. Infect. Dis. 2007;13:255–258
  23. Wulf MW, Sørum M, van Nes A, Skov R, Melchers WJ, Klaassen CH, et al. Prevalence of methicillin-resistant Staphylococcus aureus among veterinarians: an international study. Clin. Microbiol. Infect. 2008;14:29–34

☆ Results presented in part at the 19th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), Helsinki, Finland, May 16–19, 2009.

☆☆ Competing interests. The authors declare that they have no competing interests.

PII: S0732-8893(09)00369-1

doi: 10.1016/j.diagmicrobio.2009.09.006

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 217-221 , February 2010

Article Tools

* Image Usage & Resolution