Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 214-216, February 2010

Rapid identification of Propionibacterium acnes from blood cultures by fluorescence in situ hybridization

  • Sven Poppert

      Affiliations

    • Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49-40-18-20-93-78; fax: +49-40-42-81-85-12.
    • ,
  • Melanie Riecker

      Affiliations

    • Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany
    • ,
  • Andreas Essig

      Affiliations

    • Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany

Received 7 July 2009; accepted 3 September 2009. published online 08 October 2009.

Abstract 

A newly designed probe for rapid identification of Propionibacterium acnes by fluorescence in situ hybridization was evaluated using 111 isolates from subculture and showed 100% sensitivity and specificity. A sensitivity of 95% and a specificity of 100% were achieved with direct application on 55 blood cultures containing Gram-positive rods.

Keywords: FISH, In situ hybridization, Probe, Propionibacterium, Blood culture, rRNA

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PII: S0732-8893(09)00370-8

doi:10.1016/j.diagmicrobio.2009.09.007

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 214-216, February 2010

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