Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 214-216 , February 2010

Rapid identification of Propionibacterium acnes from blood cultures by fluorescence in situ hybridization

  • Sven Poppert

      Affiliations

    • Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49-40-18-20-93-78; fax: +49-40-42-81-85-12.
    • ,
  • Melanie Riecker

      Affiliations

    • Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany
    • ,
  • Andreas Essig

      Affiliations

    • Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany

Received 7 July 2009 ,Accepted 3 September 2009.

References 

  1. Alexeyev OA, Marklund I, Shannon B, Golovleva I, Olsson J, Andersson C, et al. Direct visualization of Propionibacterium acnes in prostate tissue by multicolor fluorescent in situ hybridization assay. J. Clin. Microbiol. 2007;45:3721–3728
  2. Amann RI, Binder BJ, Olson RJ, Chisholm SW, Devereux R, Stahl DA. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 1990;56:1919–1925
  3. Arnell K, Cesarini K, Lagerqvist-Widh A, Wester T, Sjolin J. Cerebrospinal fluid shunt infections in children over a 13-year period: anaerobic cultures and comparison of clinical signs of infection with Propionibacterium acnes and with other bacteria. J. Neurosurg. Pediatr. 2008;1:366–372
  4. Hiraishi A. Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett. Appl. Microbiol. 1992;15:210–213
  5. Hugenholtz P, Tyson GW, Blackall LL. Design and evaluation of 16S rRNA-targeted oligonucleotide probes for fluorescence in situ hybridization. Methods Mol. Biol. 2002;179:29–42
  6. Kempf VA, Trebesius K, Autenrieth IB. Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J. Clin. Microbiol. 2000;38:830–838
  7. Lau SK, Woo PC, Fung AM, Chan KM, Woo GK, Yuen KY. Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing. J. Med. Microbiol. 2004;53:1247–1253
  8. Lee CC, Lin WJ, Shih HI, Wu CJ, Chen PL, Lee HC, et al. Clinical significance of potential contaminants in blood cultures among patients in a medical center. J. Microbiol. Immunol. Infect. 2007;40:438–444
  9. Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar , et al. ARB: a software environment for sequence data. Nucleic Acids Res. 2004;32:1363–1371
  10. Perry AL, Lambert PA. Propionibacterium acnes. Lett. Appl. Microbiol. 2006;42:185–188
  11. Peters RP, van Agtmael MA, Simoons-Smit AM, Danner SA, Vandenbroucke-Grauls CM, Savelkoul PH. Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay. J. Clin. Microbiol. 2006;44:4186–4188
  12. Sampedro MF, Piper KE, McDowell A, Patrick S, Mandrekar JN, Rouse MS, et al. Species of Propionibacterium and Propionibacterium acnes phylotypes associated with orthopedic implants. Diagn. Microbiol. Infect. Dis. 2009;64:138–145
  13. Sohail MR, Gray AL, Baddour LM, Tleyjeh IM, Virk A. Infective endocarditis due to Propionibacterium species. Clin. Microbiol. Infect. 2009;15:387–394
  14. Sfanos KS, Isaacs WB. An evaluation of PCR primer sets used for detection of Propionibacterium acnes in prostate tissue samples. Prostate. 2008;68:1492–1495
  15. Wellinghausen N, Bartel M, Essig A, Poppert S. Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization. J. Clin. Microbiol. 2007;45:3424–3426
  16. Yamada T, Eishi Y, Ikeda S, Ishige I, Suzuki T, Takemura T, et al. In situ localization of Propionibacterium acnes DNA in lymph nodes from sarcoidosis patients by signal amplification with catalysed reporter deposition. J. Pathol. 2002;198:541–547

PII: S0732-8893(09)00370-8

doi: 10.1016/j.diagmicrobio.2009.09.007

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 214-216 , February 2010

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