Distribution of blaOXA-carrying imipenem-resistant Acinetobacter spp. in 3 hospitals in Taiwan

Kuo, Han-Yueh; Yang, Chih-Man; Lin, Ming-Feng; Cheng, Wen-Lin; Tien, Ni; Liou, Ming-Li

doi:10.1016/j.diagmicrobio.2009.09.013

« Previous Next »Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 2 , Pages 195-199, February 2010

Distribution of blaOXA-carrying imipenem-resistant Acinetobacter spp. in 3 hospitals in Taiwan

Han-Yueh Kuo
- Department of Medicine, Hsin-Chu General Hospital, Department of Health, Hsin-Chu City 30015, Taiwan
- Equal contribution to the article.
Chih-Man Yang
- Department of Laboratory, Hsin-Chu General Hospital, Department of Health, Hsin-Chu City 30015, Taiwan
- Equal contribution to the article.
Ming-Feng Lin
- Department of Medicine, Chutung Hospital, Department of Health, Hsin-Chu County 31064, Taiwan
Wen-Lin Cheng
- Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsin-Chu City 30015, Taiwan
Ni Tien
- Department of Laboratory Medicine, China Medical University Hospital, Taichung 40402, Taiwan
Ming-Li Liou
- Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsin-Chu City 30015, Taiwan
- Corresponding author. Tel.: +886-35-381183; fax: +886-35-308630.

Received 20 May 2009; accepted 22 September 2009. published online 16 October 2009.

Abstract

We investigated the molecular epidemiology and OXA-type carbapenemase genes of 83 imipenem-resistant Acinetobacter spp. collected from 2 university hospitals (hospitals A and B) and a regional hospital (hospital C) during 2007 in Taiwan. Genotyping by pulsed-field gel electrophoresis identified 51 pulsotypes. None of the pulsotypes established predominance throughout the 3 hospitals. Multiplex polymerase chain reaction of blaOXA genes showed that 100% (18/18), 91%(31/34), and 100% (31/31) of the Acinetobacter spp. collected from hospital A, B, and C, respectively, possessed blaOXA-51–like genes. None of the strains carrying blaOXA-23–like and blaOXA-24–like genes were found in hospital A. The coexistences of blaOXA-51–like/blaOXA-23–like and blaOXA-51–like/blaOXA-24–like genes detected in hospitals B and C were 26% (9/34) and 12% (4/34) and 58% (18/31) and 3% (1/31), respectively. Among blaOXA-23–like gene-carrying isolates collected from hospitals, clonal spread of strains carrying the blaOXA-23 gene was detected in the regional hospital but not the other 2 university hospitals. The results suggest that interhospital dissemination of imipenem-resistant Acinetobacter spp. was not found in these hospitals. The increasing percentage of OXA-23 in OXA-type carbapenemases in Acinetobacter spp. from the regional hospitals to medical centers deserves further attention in Taiwan.