Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 326-328, March 2010

Plasmid-mediated carbapenem-hydrolyzing enzyme KPC-2 and ArmA 16S rRNA methylase conferring high-level aminoglycoside resistance in carbapenem-resistant Enterobacter cloacae in China

Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, People's Republic of China

Received 30 May 2009; accepted 4 October 2009. published online 11 November 2009.

Abstract 

The emergence and spread of carbapenem-resistant Enterobacteriaceae in the world are a major concern. We investigated 5 isolates of Enterobacter cloacae that were resistant to all clinically available antimicrobial agents, except polymyxin B. The MICs of imipenem and aminoglycosides were >32 and >256 mg/L, respectively. All of the isolates produced 5 extended-spectrum beta-lactamase (ESBLs) with pIs of 5.4 (TEM-1), 6.7 (KPC-2), 8.2 (SHV-12), 8.4 (CTX-M-14), and ArmA 16S rRNA methylase. blaKPC-2 was located on a large nonconjugative plasmid, whereas armA was located on another conjugative plasmid. Although carbapenem-resistant Enterobacteriaceae remains rare, the emergence of this group of organism merits monitoring.

Keywords: Carbapenem, Aminoglycoside, Resistance, 16S rRNA methylase, Enterobacter cloacae

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PII: S0732-8893(09)00413-1

doi:10.1016/j.diagmicrobio.2009.10.003

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 326-328, March 2010

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