Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 261-267 , March 2010

Evaluation of a real-time polymerase chain reaction assay for the laboratory diagnosis of giardiasis

  • Adriana Calderaro

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39-0521-988885; fax: +39-0521-993620.
  • ,
  • Chiara Gorrini

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
  • ,
  • Sara Montecchini

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
  • ,
  • Simona Peruzzi

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
  • ,
  • Giovanna Piccolo

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
  • ,
  • Sabina Rossi

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
  • ,
  • Franco Gargiulo

      Affiliations

    • Department of Experimental and Applied Medicine, Institute of Microbiology, Spedali Civili, University of Brescia, 25123 Brescia, Italy
  • ,
  • Nino Manca

      Affiliations

    • Department of Experimental and Applied Medicine, Institute of Microbiology, Spedali Civili, University of Brescia, 25123 Brescia, Italy
  • ,
  • Giuseppe Dettori

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
  • ,
  • Carlo Chezzi

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy

Received 3 April 2009 ,Accepted 6 October 2009.

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 AC and CG conceived and designed the experiments. AC, CG, SM, SP, and GP performed the experiments. SM, FG, and NM performed the sequencing. CG, SM, and GP did the sequence analysis. AC, CG, and SM wrote the paper. AC, and SR performed the conventional parasitologic diagnostic methods. GD and CC conducted the scientific supervision of the manuscript.

PII: S0732-8893(09)00414-3

doi: 10.1016/j.diagmicrobio.2009.10.004

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 261-267 , March 2010