« Previous
Next »
Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3
, Pages 261-267
, March 2010
Evaluation of a real-time polymerase chain reaction assay for the laboratory diagnosis of giardiasis
References
- . Sequence analysis of the β-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int. J. Parasitol. 2002;32:1023–1030
- . Unravelling Cryptosporidium and Giardia epidemiology. Trends Parasitol. 2005;21:430–437
- . Molecular epidemiology of giardiasis. Mol. Biochem. Parasitol. 2008;160:75–80
- . Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. Int. J. Parasitol. 2008;38:1523–1531
- . Entamoeba histolytica and Entamoeba dispar: comparison of two PCR assay for diagnosis in a non-endemic setting. Trans. Roy. Soc. Trop. Med. Hyg. 2006;100:450–457
- . Outbreak of Entamoeba histolytica and Giardia lamblia infections in travellers returning from the tropics. Infection. 1992;20:78–82
- . Giardiasis: the clinical spectrum, diagnosis and therapy. Pediatr Infect Dis. 1986;5:131–138
- . Immune response to Giardia duodenalis. Clin. Microbiol. Rev. 2000;13:25–54
- . Giardia lamblia key points-laboratory diagnosis. In: Diagnostic medical parasitology. 3:Washington, DC: ASM PRESS; 1997;p. 42–44
- . Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens. J. Clin. Microbiol. 1997;35:1526–1529
- . Molecular characterization of human isolates of Giardia duodenalis from Ethiopia. Acta Trop. 2007;102:92–99
- . Giardia and Cryptosporidium and public health: the epidemiological scenario from the Italian perspective. Parasitol. Res. 2007;101:1169–1182
- . Giardia assemblage A infection and diarrhea in Bangladesh. J. Infect. Dis. 2005;192:2171–2173
- . Multiplex real-time PCR assay for detection of Entamoeba histolytica, Giardia intestinalis and Cryptosporidium spp. Am. J. Trop. Med. Hyg. 2007;76:713–717
- . Human giardiasis: genotype linked differences in clinical symptomatology. Int. J. Parasitol. 2001;31:822–826
- . Genotyping of Giardia duodenalis from humans and dogs from Mexico using a β-giardin nested-polymerase chain reaction assay. J. Parasitol. 2005;91:203–205
- . Genetic heterogeneity at the β-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes. Int. J. Parasitol. 2005;35:207–213
- . Prevalence of intestinal parasites in the area of Parma during the year 2005. Acta Biomed. 2006;77:147–151
- . Correlation between genotype of Giardia duodenalis and diarrhoea. Int. J. Parasitol. 2002;32:229–231
- . Correlation between the presence of symptoms and the Giardia duodenalis genotype. Eur. J. Clin. Microbiol. Infect. Dis. 2008;27:81–83
- . Comparison of microscopy, real-time PCR and a rapid immunoassay for the detection of Giardia lamblia in human stool specimens. Clin. Microbiol. Infect. 2007;13:1186–1191
- . Real-time PCR in clinical microbiology: verification, validation and contamination control. Clin. Microbiol. Newslett. 2007;29:87–95
- . Cryptosporidium and Giardia as foodborne zoonoses. Vet. Parasitol. 2007;149:29–40
- . Nomenclature and genetic groupings of Giardia infecting mammals. Parasitol. Today. 2000;16:210–213
- . Real-time PCR for the detection of Giardia lamblia. Mol. Cell. Probes. 2003;17:223–225
- . Simultaneous detection of Entamoeba histolytica, Giardia intestinalis and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. J. Clin. Microbiol. 2004;42:1220–1223
☆ AC and CG conceived and designed the experiments. AC, CG, SM, SP, and GP performed the experiments. SM, FG, and NM performed the sequencing. CG, SM, and GP did the sequence analysis. AC, CG, and SM wrote the paper. AC, and SR performed the conventional parasitologic diagnostic methods. GD and CC conducted the scientific supervision of the manuscript.
PII: S0732-8893(09)00414-3
doi: 10.1016/j.diagmicrobio.2009.10.004
© 2010 Elsevier Inc. All rights reserved.
« Previous
Next »
Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3
, Pages 261-267
, March 2010
