Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 235-240 , March 2010

Determination of capsulation status in Haemophilus influenzae by multiplex polymerase chain reaction

Received 4 June 2009 ,Accepted 6 October 2009.

References 

  1. Bokermann S, Zanella RC, Lemos AP, de Andrade AL, Brandileone MC. Evaluation of methodology for serotyping invasive and nasopharyngeal isolates of Haemophilus influenzae in the ongoing surveillance in Brazil. J. Clin. Microbiol. 2003;41:5546–5550
  2. Cerquetti M, Cardines R, Giufrè M, Sofia T, D'Ambrosio F, Mastrantonio P, et al. Genetic diversity of invasive strains of Haemophilus influenzae type b before and after introduction of the conjugate vaccine in Italy. Clin. Infect. Dis. 2006;43:317–319
  3. Edwards MC, Gibbs RA. Multiplex PCR: advantages, development, and applications. PCR Methods Appl. 1994;3:S65–S75
  4. Falla TJ, Anderson EC, Chappell MM, Slack MP, Crook DW. Cross-reaction of spontaneous capsule-deficient Haemophilus influenzae type b mutants with type-specific antisera. Eur. J. Clin. Microbiol. Infect. Dis. 1993;12:147–148
  5. Falla TJ, Crook DW, Brophy LN, Maskell D, Kroll JS, Moxon ER. PCR for capsular typing of Haemophilus influenzae. J. Clin.Microbiol. 1994;32:2382–2386
  6. Gonin P, Lorange M, Delage G. Performance of a multiplex PCR for the determination of Haemophilus influenzae capsular types in the clinical microbiology laboratory. Diagn. Microbiol. Infect. Dis. 2000;37:1–4
  7. Harrison A, Dyer DW, Gillaspy A, Ray WC, Mungur R, Carson MB, et al. Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20. J. Bacteriol. 2005;187:4627–4636
  8. Himmelreich CA, Barenkamp SJ, Storch GA. Comparison of methods for serotyping isolates of Haemophilus influenzae. J. Clin. Microbiol. 1985;21:158–160
  9. Hoiseth SK, Connelly CJ, Moxon ER. Genetics of spontaneous, high-frequency loss of b capsule expression in Haemophilus influenzae. Infect. Immun. 1985;49:389–395
  10. Hoiseth SK, Moxon ER, Silver RP. Genes involved in Haemophilus influenzae type b capsule expression are part of an 18-kilobase tandem duplication. Proc. Natl. Acad. Sci. U. S. A. 1986;83:1106–1110
  11. Kroll JS, Moxon ER. Capsulation and gene copy number at the cap locus of Haemophilus influenzae type b. J. Bacteriol. 1988;170:859–864
  12. Kroll JS, Moxon ER. Genetic and phenotypic variation in the capsulation and virulence of culture collection strains of Haemophilus influenzae type b. Microb. Pathog. 1989;7:449–457
  13. Kroll JS, Hopkins I, Moxon ER. Capsule loss in H. influenzae type b occurs by recombination-mediated disruption of a gene essential for polysaccharide export. Cell. 1988;53:347–356
  14. Kroll JS, Loynds B, Brophy LN, Moxon ER. The bex locus in encapsulated Haemophilus influenzae: a chromosomal region involved in capsule polysaccharide export. Mol. Microbiol. 1990;4:1853–1862
  15. Kroll JS, Ely S, Moxon ER. Capsular typing of Haemophilus influenzae with a DNA probe. Mol. Cell. Probes. 1991;5:375–379
  16. Kroll JS, Loynds BM, Moxon ER. The Haemophilus influenzae capsulation gene cluster: a compound transposon. Mol. Microbiol. 1991;5:1549–1560
  17. Kroll JS, Moxon ER, Loynds BM. An ancestral mutation enhancing the fitness and increasing the virulence of Haemophilus influenzae type b. J. Infect. Dis. 1993;168:172–176
  18. LaClaire LL, Tondella ML, Beall DS, Noble CA, Raghunathan PL, Rosenstein NE, et al. Identification of Haemophilus influenzae serotypes by standard slide agglutination serotyping and PCR-based capsule typing. J. Clin. Microbiol. 2003;41:393–396
  19. Nizet V, Colina KF, Almquist JR, Rubens CE, Smith AL. A virulent nonencapsulated Haemophilus influenzae. J. Infect. Dis. 1996;173:180–186
  20. Peltola H. Worldwide Haemophilus influenzae type b disease at the beginning of the 21st century: global analysis of the disease burden 25 years after the use of the polysaccharide vaccine and a decade after the advent of conjugates. Clin. Microbiol. Rev. 2000;13:302–317
  21. Pittman M. Variation and type specificity in the bacterial species H. influenzae. J. Exp. Med. 1931;53:471–492
  22. Sam IC, Smith M. Failure to detect capsule gene bexA in Haemophilus influenzae types e and f by real-time PCR due to sequence variation within probe binding sites. J. Med. Microbiol. 2005;54(Pt. 5):453–455
  23. Satola SW, Collins JT, Napier R, Farley MM. Capsule gene analysis of invasive Haemophilus influenzae: accuracy of serotyping and prevalence of IS1016 among nontypeable isolates. J. Clin. Microbiol. 2007;45:3230–3238
  24. Shively RG, Shigei JT, Peterson EM, de la Maza LM. Typing of Haemophilus influenzae by coagglutination and conventional slide agglutination. J. Clin. Microbiol. 1981;14:706–708
  25. Slack MP. Invasive Haemophilus influenzae disease: the impact of Hib immunisation. J. Med. Microbiol. 1995;42:75–77
  26. van Ketel RJ, de Wever B, van Alphen L. Detection of Haemophilus influenzae in cerebrospinal fluids by polymerase chain reaction DNA amplification. J. Med. Microbiol. 1990;33:271–276
  27. Wilcox KW, Smith HO. Isolation and characterization of mutants of Haemophilus influenzae deficient in an adenosine 5′-triphosphate-dependent deoxyribonuclease activity. J. Bacteriol. 1975;122:443–453
  28. Zhou J, Law DK, Sill ML, Tsang RS. Nucleotide Sequence Diversity of the bexA gene in serotypeable Haemophilus influenzae strains recovered from invasive disease patients in Canada. J. Clin. Microbiol. 2007;45:1996–1999

 Supported in part by Public Health Service grant AI 44002 from the National Institute for Allergy and Infectious Disease.

PII: S0732-8893(09)00415-5

doi: 10.1016/j.diagmicrobio.2009.10.005

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 235-240 , March 2010