Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 248-252, March 2010

Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on ϕETA prophage polymorphisms

  • Pavla Holochová

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic
    • ,
  • Vladislava Růžičková

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic
    • Corresponding Author InformationCorresponding author. Tel.: +420-5-49496827; fax: +420-5-49492570.
    • ,
  • Lucie Dostálová

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic
    • ,
  • Roman Pantůček

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic
    • ,
  • Petr Petráš

      Affiliations

    • Reference Laboratory for Staphylococci, National Institute of Public Health, 100 42 Prague, Czech Republic
    • ,
  • Jiří Doškař

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic

Received 9 June 2009; accepted 7 October 2009. published online 11 November 2009.

Abstract 

The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various ϕETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control.

Keywords: Staphylococcus aureus, Pemphigus neonatorum, Exfoliative toxin A, Prophages, Multiplex PCR

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PII: S0732-8893(09)00418-0

doi:10.1016/j.diagmicrobio.2009.10.008

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 248-252, March 2010

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