Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 248-252 , March 2010

Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on ϕETA prophage polymorphisms

  • Pavla Holochová

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic
    • ,
  • Vladislava Růžičková

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic
    • Corresponding Author InformationCorresponding author. Tel.: +420-5-49496827; fax: +420-5-49492570.
    • ,
  • Lucie Dostálová

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic
    • ,
  • Roman Pantůček

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic
    • ,
  • Petr Petráš

      Affiliations

    • Reference Laboratory for Staphylococci, National Institute of Public Health, 100 42 Prague, Czech Republic
    • ,
  • Jiří Doškař

      Affiliations

    • Faculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic

Received 9 June 2009 ,Accepted 7 October 2009.

References 

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  2. Endo Y, Yamada T, Matsunaga K, Hayakawa Y, Kaidoh T, Takeuchi S. Phage conversion of exfoliative toxin A in Staphylococcus aureus isolated from cows with mastitis. Vet. Microbiol. 2003;96:81–90
  3. Henegariu O, Heerema NA, Dlouhy SR, Vance GH, Vogt PH. Multiplex PCR: critical parameters and step-by-step protocol. Biotechniques. 1997;23:504–511
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  5. Ladhani S, Joannou CL, Lochrie DP, Evans RW, Poston SM. Clinical, microbial, and biochemical aspects of the exfoliative toxins causing staphylococcal scalded-skin syndrome. Clin. Microbiol. Rev. 1999;12:224–242
  6. Mackenzie A, Johnson W, Heyes B, Norrish B, Jamieson F. A prolonged outbreak of exfoliative toxin A-producing Staphylococcus aureus in a newborn nursery. Diagn. Microbiol. Infect. Dis. 1995;21:69–75
  7. Mehrotra M, Wang G, Johnson WM. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxin shock syndrome toxin 1, and methicillin resistance. J. Clin. Microbiol. 2000;38:1032–1035
  8. Pantůček R, Götz F, Doškař J, Rosypal S. Genomic variability of Staphylococcus aureus and the other coagulase-positive Staphylococcus species estimated by macrorestriction analysis using pulsed-field gel electrophoresis. Int. J. Syst. Bacteriol. 1996;46:216–222
  9. Pantůček R, Doškař J, Růžičková V, Kašpárek P, Oráčová E, Kvardová V, et al. Identification of bacteriophage types and their carriage in Staphylococcus aureus. Arch. Virol. 2004;149:1689–1703
  10. Plano LR. Staphylococcus aureus exfoliative toxins: how they cause disease. J. Invest. Dermatol. 2004;122:1070–1077
  11. Růžičková V, Pantůček R, Petráš P, Doškař J, Sedláček I, Rosypal S. Molecular typing of exfoliative toxin-producing Staphylococcus aureus strains involved in epidermolytic infections. Int. J. Med. Microbiol. 2003;292:541–545
  12. Růžičková V, Voller J, Pantůček R, Petráš P, Doškař J. Multiplex PCR for detection of three exfoliative toxin serotype genes in Staphylococcus aureus. Folia Microbiol. 2005;50:499–502
  13. Růžičková V, Karpíšková R, Pantůček R, Pospíšilová M, Černíková P, Doškař J. Genotype analysis of enterotoxin H-positive Staphylococcus aureus strains isolated from food samples in the Czech Republic. Int. J. Food. Microbiol. 2008;121:60–65
  14. Saiman L, Jakob K, Holmes KW, Whittier S, Garzon MC, Rago JV, et al. Molecular epidemiology of staphylococcal scalded skin syndrome in premature infants. Pediatr. Infect. Dis. 1998;17:329–334
  15. Sato H, Matsumori Y, Tanabe T, Saito H, Shimizu A, Kawano J. A new type of staphylococcal exfoliative toxin from a Staphylococcus aureus strain isolated from a horse with phlegmon. Infect. Immun. 1994;62:3780–3785
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  17. Yamaguchi T, Hayashi T, Takami H, Nakasone K, Ohnishi M, Nakayama K, et al. Phage conversion of exfoliative toxin A production in Staphylococcus aureus. Mol. Microbiol. 2000;38:694–705
  18. Yamaguchi T, Nishifuji K, Sasaki M, Fudaba Y, Aepfelbacher M, Takata T, et al. Identification of the Staphylococcus aureus etd pathogenicity island which encodes a novel exfoliative toxin, ETD, and EDIN-B.. Infect. Immun. 2002;70:5835–5845
  19. Yoshizawa Y, Sakurada J, Sakurai S, Machida K, Kondo I, Masuda S. An exfoliative toxin A-converting phage isolated from Staphylococcus aureus strain ZM. Microbiol. Immunol. 2000;44:189–191

PII: S0732-8893(09)00418-0

doi: 10.1016/j.diagmicrobio.2009.10.008

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 248-252 , March 2010

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