Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 268-273 , March 2010

Detection of Histoplasma capsulatum DNA in human samples by real-time polymerase chain reaction

  • Stephane Simon

      Affiliations

    • Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie Médicale, équipe EA3593, UFR de Médecine, Université des Antilles et de la Guyane, French Guiana
    • ,
  • Vincent Veron

      Affiliations

    • Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie Médicale, équipe EA3593, UFR de Médecine, Université des Antilles et de la Guyane, French Guiana
    • ,
  • Rachida Boukhari

      Affiliations

    • Laboratoire de Biologie Médicale, Centre hospitalier de l'Ouest Guyanais Frank Joly, 97320 Saint Laurent du Maroni, Guyane française
    • ,
  • Denis Blanchet

      Affiliations

    • Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie Médicale, équipe EA3593, UFR de Médecine, Université des Antilles et de la Guyane, French Guiana
    • ,
  • Christine Aznar

      Affiliations

    • Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie Médicale, équipe EA3593, UFR de Médecine, Université des Antilles et de la Guyane, French Guiana
    • Corresponding Author InformationCorresponding author. Service de Parasitologie-Mycologie Médicale (LHUPM), Centre Hospitalier Andrée Rosemon, BP 6006, rue des flamboyants, Cayenne, French Guiana. Tel.: +594-594-29-61-32; fax: +594-594-29-34-13.

Received 25 June 2009 ,Accepted 9 October 2009.

References 

  1. Benevides CF, Duraes RO, Aquino B, Schiavon Lde L, Narciso-Schiavon JL, Buzzoleti Fda C. Bilateral adrenal histoplasmosis in an immunocompetent man. Rev. Soc. Bras. Med. Trop. 2007;40:230–233
  2. Bialek R, Ernst F, Dietz K, Najvar LK, Knobloch J, Graybill JR, et al. Comparison of staining methods and a nested PCR assay to detect Histoplasma capsulatum in tissue sections. Am. J. Clin. Pathol. 2002;117:597–603
  3. Bialek R, Feucht A, Aepinus C, Just-Nubling G, Robertson VJ, Knobloch J, et al. Evaluation of two nested PCR assays for detection of Histoplasma capsulatum DNA in human tissue. J. Clin. Microbiol. 2002;40:1644–1647
  4. Bracca A, Tosello ME, Girardini JE, Amigot SL, Gomez C, Serra E. Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples. J. Clin. Microbiol. 2003;41:1753–1755
  5. Buitrago MJ, Berenguer J, Mellado E, Rodriguez-Tudela JL, Cuenca-Estrella M. Detection of imported histoplasmosis in serum of HIV-infected patients using a real-time PCR-based assay. Eur. J. Clin. Microbiol. Infect. Dis. 2006;25:665–668
  6. Couppie P, Aznar C, Carme B, Nacher M. American histoplasmosis in developing countries with a special focus on patients with HIV: diagnosis, treatment, and prognosis. Curr. Opin. Infect. Dis. 2006;19:443–449
  7. Darling ST. A protozoan general infection producing pseudotubercles in the lungs and focal necrosis in the liver, spleen and lymph nodes. J. Am. Med. Assoc. 1906;46:1283–1285
  8. Floch H. L'histoplasmose de S. T. Darling (II). Sur sa présence en Guyane française. Arch. Inst. Pasteur. Guyane Fr. Inini. 1953;283:1–11
  9. Goodman NL, Larsh HW. Environmental factors and growth of Histoplasma capsulatum in soil. Mycopathol. Mycol. Appl. 1967;33:145–156
  10. Imhof A, Schaer C, Schoedon G, Schaer DJ, Walter RB, Schaffner A, et al. Rapid detection of pathogenic fungi from clinical specimens using LightCycler real-time fluorescence PCR. Eur. J. Clin. Microbiol. Infect. Dis. 2003;22:558–560
  11. Lewden C, Sobesky M, Cabie A, Couppie P, Boulard F, Bissuel F, et al. Causes of death among HIV infected adults in French Guyana and the French West Indies in the era of highly active antiretroviral therapy (HAART). Med. Mal. infect. 2004;34:286–292
  12. Martagon-Villamil J, Shrestha N, Sholtis M, Isada CM, Hall GS, Bryne T, et al. Identification of Histoplasma capsulatum from culture extracts by real-time PCR. J. Clin. Microbiol. 2003;41:1295–1298
  13. Maubon D, Simon S, Aznar C. Histoplasmosis diagnosis using a polymerase chain reaction method. Application on human samples in French Guiana, South America. Diagn. Microbiol. Infect. Dis. 2007;58:441–444
  14. Schestatsky P, Chedid MF, Amaral OB, Unis G, Oliveira FM, Severo LC. Isolated central nervous system histoplasmosis in immunocompetent hosts: a series of 11 cases. Scand. J. Infect. Dis. 2006;38:43–48
  15. Washington L, Palacio D. Imaging of bacterial pulmonary infection in the immunocompetent patient. Semin. Roentgenol. 2007;42:122–145
  16. Wheat LJ. Current diagnosis of histoplasmosis. Trends Microbiol. 2003;11:488–494
  17. Wheat LJ. Histoplasmosis: a review for clinicians from non-endemic areas. Mycoses. 2006;49:274–282

PII: S0732-8893(09)00420-9

doi: 10.1016/j.diagmicrobio.2009.10.010

Diagnostic Microbiology & Infectious Disease
Volume 66, Issue 3 , Pages 268-273 , March 2010

Article Tools

* Image Usage & Resolution