Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 1 , Pages 37-46 , May 2010

Systematic interpretation of molecular beacon polymerase chain reaction for identifying rpoB mutations in Mycobacterium tuberculosis isolates with mixed resistant and susceptible bacteria

  • Diana I. Gomez

      Affiliations

    • The University of Texas Health Science Center Houston, School of Public Health at Brownsville, Brownsville, TX 78520, USA
    • ,
  • Susan P. Fisher-Hoch

      Affiliations

    • The University of Texas Health Science Center Houston, School of Public Health at Brownsville, Brownsville, TX 78520, USA
    • ,
  • Andrea S. Bordt

      Affiliations

    • The University of Texas Health Science Center Houston, School of Public Health at Brownsville, Brownsville, TX 78520, USA
    • ,
  • Teresa N. Quitugua

      Affiliations

    • Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA
    • ,
  • Jaime Robledo

      Affiliations

    • Bacteriology and Mycobacteriology Unit, Corporación para Investigaciones Biológicas, Medellin, Colombia
    • ,
  • Nataly Alvarez

      Affiliations

    • Bacteriology and Mycobacteriology Unit, Corporación para Investigaciones Biológicas, Medellin, Colombia
    • ,
  • Nidia Correa

      Affiliations

    • Bacteriology and Mycobacteriology Unit, Corporación para Investigaciones Biológicas, Medellin, Colombia
    • ,
  • Joseph B. McCormick

      Affiliations

    • The University of Texas Health Science Center Houston, School of Public Health at Brownsville, Brownsville, TX 78520, USA
    • ,
  • Blanca I. Restrepo

      Affiliations

    • The University of Texas Health Science Center Houston, School of Public Health at Brownsville, Brownsville, TX 78520, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-956-882-5172; fax: +1-956-882-5152.

Received 15 July 2009 ,Accepted 11 December 2009.

References 

  1. Ahmad S, Araj GF, Akbar PK, Fares E, Chugh TD, Mustafa AS. Characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from the Middle East. Diagn. Microbiol. Infect. Dis. 2000;38:227–232
  2. Ahmad S, Mokaddas E. The occurrence of rare rpoB mutations in rifampicin-resistant clinical Mycobacterium tuberculosis isolates from Kuwait. Int. J. Antimicrob. Agents. 2005;26:205–212
  3. Bartfai Z, Somoskovi A, Kodmon C, Szabo N, Puskas E, Kosztolanyi L, et al. Molecular characterization of rifampin-resistant isolates of Mycobacterium tuberculosis from Hungary by DNA sequencing and the line probe assay. J. Clin. Microbiol. 2001;39:3736–3739
  4. Cavusoglu C, Hilmioglu S, Guneri S, Bilgic A. Characterization of rpoB mutations in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. J. Clin. Microbiol. 2002;40:4435–4438
  5. Cooksey RC, Morlock GP, Glickman S, Crawford JT. Evaluation of a line probe assay kit for characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from New York City. J. Clin. Microbiol. 1997;35:1281–1283
  6. Eickbush TH, Moudrianakis EN. The compaction of DNA helices into either continuous supercoils or folded-fiber rods and toroids. Cell. 1978;13:295–306
  7. El-Hajj HH, Marras SA, Tyagi S, Kramer FR, Alland D. Detection of rifampin resistance in Mycobacterium tuberculosis in a single tube with molecular beacons. J. Clin. Microbiol. 2001;39:4131–4137
  8. Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res. 1996;6:986–994
  9. Hirano K, Abe C, Takahashi M. Mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis strains isolated mostly in Asian countries and their rapid detection by line probe assay. J. Clin. Microbiol. 1999;37:2663–2666
  10. Hoek KG, Gey van Pittius NC, Moolman-Smook H, Carelse-Tofa K, Jordaan A, van der Spuy GD, et al. Fluorometric assay for testing rifampin susceptibility of Mycobacterium tuberculosis complex. J. Clin. Microbiol. 2008;46:1369–1373
  11. Jureen P, Engstrand L, Eriksson S, Alderborn A, Krabbe M, Hoffner SE. Rapid detection of rifampin resistance in Mycobacterium tuberculosis by pyrosequencing technology. J. Clin. Microbiol. 2006;44:1925–1929
  12. Kent PT, Kubica GP. Public health mycobacteriology: a guide for the level III laboratory. Atlanta, GA: Center for Disease Control and Prevention; 1985;
  13. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, et al. Clustal W and Clustal X version 2.0. Bioinformatics. 2007;23:2947–2948
  14. Lin S-YG, Probert W, Lo M, Desmond E. Rapid detection of isoniazid and rifampin resistance mutations in Mycobacterium tuberculosis complex from cultures or smear-positive sputa by use of molecular beacons. J. Clin. Microbiol. 2004;42:4204–4208
  15. Matsiota-Bernard P, Vrioni G, Marinis E. Characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates from Greece. J. Clin. Microbiol. 1998;36:20–23
  16. McCammon MT, Gillette JS, Thomas DP, Ramaswamy SV, Graviss EA, Kreiswirth BN, et al. Detection of rpoB mutations associated with rifampin resistance in Mycobacterium tuberculosis using denaturing gradient gel electrophoresis. Antimicrob. Agents Chemother. 2005;49:2200–2209
  17. Nikolayevskyy VV, Brown TJ, Bazhora YI, Asmolov AA, Balabanova YM, Drobniewski FA. Molecular epidemiology and prevalence of mutations conferring rifampicin and isoniazid resistance in Mycobacterium tuberculosis strains from the southern Ukraine. Clin. Microbiol. Infect. 2007;13:129–138
  18. Ozkutuk N, Gazi H, Surucuoglu S, Gunduz A, Ozbakkaloglu B. Characterization of rpoB mutations by line probe assay in rifampicin-resistant Mycobacterium tuberculosis clinical isolates from the Aegean region in Turkey. Jpn. J. Infect. Dis. 2007;60:211–213
  19. Palomino JC. Newer diagnostics for tuberculosis and multi-drug resistant tuberculosis. Curr. Opin. Pulm. Med. 2006;12:172–178
  20. Piatek AS, Telenti A, Murray MR, El-Hajj H, Jacobs WR, Kramer FR, et al. Genotypic analysis of Mycobacterium tuberculosis in two distinct populations using molecular beacons: implications for rapid susceptibility testing. Antimicrob. Agents Chemother. 2000;44:103–110
  21. Piatek AS, Tyagi S, Pol AC, Telenti A, Miller LP, Kramer FR, et al. Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis. Nat. Biotechnol. 1998;16:359–363
  22. Quitugua TN, Seaworth BJ, Weis SE, Taylor JP, Gillette JS, Rosas II, et al. Transmission of drug-resistant tuberculosis in Texas and Mexico. J. Clin. Microbiol. 2002;40:2716–2724
  23. Robledo J, Mejia GI, Paniagua L, Martin A, Guzman A. Rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis by the direct thin-layer agar method. Int. J. Tuberc. Lung Dis. 2008;12:1482–1484
  24. Somoskovi A, Parsons LM, Salfinger M. The molecular basis of resistance to isoniazid, rifampin, and pyrazinamide in Mycobacterium tuberculosis. Respir. Res. 2001;2:164–168
  25. Stop TB Partnership and World Health Organization . The global plan to stop TB, 2006–2015. Geneva: Stop TB Partnership; World Health Organization; 2006;http://www.stoptb.org/globalplanWHO/HTM/STB/2006.35
  26. Stop TB Partnership and World Health Organization . New laboratory diagnostic tools for tuberculosis control. http://www.finddiagnostics.org/export/sites/default/resource-centre/reports_brochures/docs/New_Lab_Diagnostic_Tools_for_TB.pdf2008;WHO/HTM/STB/2008.49
  27. Tyagi S, Kramer FR. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 1996;14:303–308
  28. van Soolingen D, Hermans PW, de Haas PE, Soll DR, van Embden JD. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J. Clin. Microbiol. 1991;29:2578–2586
  29. Varma-Basil M, El-Hajj H, Colangeli R, Hazbon MH, Kumar S, Bose M, et al. Rapid detection of rifampin resistance in Mycobacterium tuberculosis isolates from India and Mexico by a molecular beacon assay. J. Clin. Microbiol. 2004;42:5512–5516
  30. World Health Organization . Anti-tuberculosis drug resistance in the world. Fourth Global Report. http://www.who.int/tb/publications/2008/drs_report4_26feb08.pdf2008;WHO/HTM/TB/2008.394
  31. World Health Organization . Global tuberculosis control: surveillance, planning, financing. http://www.who.int/tb/publications/global_report/2009/en/index.html2009;1-1-2003
  32. World Health Organization . Global tuberculosis control: epidemiology, strategy, financing: WHO report 2009. Geneva: World Health Organization; 2009;http://www.who.int/tb/publications/global_report/2009/en/index.htmlWHO/HTM/TB/2009.411

PII: S0732-8893(09)00484-2

doi: 10.1016/j.diagmicrobio.2009.12.007

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 1 , Pages 37-46 , May 2010

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