Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 1 , Pages 47-51 , May 2010

Use of the “RAM” susceptibility testing method for rapid detection of clarithromycin resistance in the Mycobacterium avium complex

  • Victor Flauta

      Affiliations

    • Division of Medical Microbiology, Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA
    • ,
  • Gerard Osterhout

      Affiliations

    • MIDI, Incorporated, 125 Sandy Drive, Newark, DE 19713, USA
    • ,
  • Brandon Ellis

      Affiliations

    • Division of Medical Microbiology, Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA
    • ,
  • Michael Grayson

      Affiliations

    • Division of Medical Microbiology, Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA
    • ,
  • Myron Sasser

      Affiliations

    • MIDI, Incorporated, 125 Sandy Drive, Newark, DE 19713, USA
    • ,
  • Sam Cohen

      Affiliations

    • ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    • ,
  • Melissa Sander

      Affiliations

    • Division of Medical Microbiology, Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA
    • ,
  • Kim Dionne

      Affiliations

    • Division of Medical Microbiology, Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA
    • ,
  • Karen Carroll

      Affiliations

    • Division of Medical Microbiology, Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA
    • ,
  • Nicole Parrish

      Affiliations

    • Division of Medical Microbiology, Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA
    • Corresponding Author InformationCorresponding author. Johns Hopkins University, Baltimore, MD 21287, USA. Tel.: +1-410-955-5077; fax: +1-410-614-8087.

Received 3 August 2009 ,Accepted 11 December 2009.

References 

  1. Butler WR, Guthertz LS. Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species. Clin. Microbiol. Rev. 2001;14:704–726
  2. Gilpin C, Herron K, Shipp B, Fraser R, Coulter C. Comparison of clarithromycin susceptibility of Mycobacterium avium complex using the NCCLS BACTEC™ 460 method and the BACTEC™ MGIT 960. In: Abstr. 105th general meeting of the American Society for Microbiology, Atlanta, Georgia. 2005;
  3. Griffith DE. Therapy of nontuberculous mycobacterial disease. Curr. Opin. Infec. Dis. 2007;20:198–203
  4. Griffith DE, Brown-Elliott BA, Langsjoen B, Zhang Y, Pan X, Girard W, et al. Clinical and molecular analysis of macrolide resistance in Mycobacterium avium complex lung disease. Am. J. Respir. Crit. Care Med. 2006;174:928–934
  5. Griffith DE, Aksamit T, Brown-Elliot B, Catanzaro A, Daley C, Gordin F, et al. An official ATS statement: diagnosis, treatment and prevention of nontuberculous mycobacteria. Am. J. Respir. Crit. Care Med. 2007;175:367–416
  6. Jost KC, Dunbar DF, Barth SS, Headley VL, Elliott LB. Identification of Mycobacterium tuberculosis and M. avium complex directly from smear-positive sputum specimens and BACTEC 12B cultures by high-performance liquid chromatography with fluorescence detection and computer-driven pattern recognition models. J. Clin. Microbiol. 1995;33:1270–1277
  7. Karakousis PC, Moore RD, Chaisson RE. Mycobacterium avium complex in patients with HIV infection in the era of highly active antiretroviral therapy. Lancet Infect. Dis. 2004;4:557–565
  8. Parrish N, Osterhout G, Dionne K, Sweeney A, Kwiatkowski N, Carroll K, et al. Rapid, standardized method for determination of Mycobacterium tuberculosis drug susceptibility by use of mycolic acid analysis. J. Clin. Microbiol. 2007;45:3915–3920
  9. Piersimoni C, Nista D, Bornigia S, deSio G. Evaluation of a new method for rapid drug susceptibility testing of Mycobacterium avium complex isolates by using the mycobacteria growth indicator tube. J. Clin. Microbiol. 1998;36:64–67
  10. Woods G, Williams-Bouyer N, Wallace RJ, Brown-Elliot BA, Witebsky FG, Conville PS, et al. Multisite reproducibility of results obtained by two broth dilution methods for susceptibility testing of Mycobacterium avium complex. J. Clin. Microbiol. 2003;41:627–631

PII: S0732-8893(09)00485-4

doi: 10.1016/j.diagmicrobio.2009.12.008

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 1 , Pages 47-51 , May 2010

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