Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 1 , Pages 15-21 , May 2010

The development of a selective medium for the Brucella abortus strains and its comparison with the currently recommended and used medium

  • Moon Her

      Affiliations

    • OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Institute, National Veterinary Research and Quarantine Service (NVRQS), Anyang, Gyeonggi, 430-824, Republic of Korea
    • Corresponding Author InformationCorresponding author. Tel.: +82-31-467-1776; fax: +82-31-467-1778.
  • ,
  • Dong-Hee Cho

      Affiliations

    • OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Institute, National Veterinary Research and Quarantine Service (NVRQS), Anyang, Gyeonggi, 430-824, Republic of Korea
  • ,
  • Sung-Il Kang

      Affiliations

    • OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Institute, National Veterinary Research and Quarantine Service (NVRQS), Anyang, Gyeonggi, 430-824, Republic of Korea
  • ,
  • Yun-Sang Cho

      Affiliations

    • OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Institute, National Veterinary Research and Quarantine Service (NVRQS), Anyang, Gyeonggi, 430-824, Republic of Korea
  • ,
  • In-Yeong Hwang

      Affiliations

    • OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Institute, National Veterinary Research and Quarantine Service (NVRQS), Anyang, Gyeonggi, 430-824, Republic of Korea
  • ,
  • You-Chan Bae

      Affiliations

    • OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Institute, National Veterinary Research and Quarantine Service (NVRQS), Anyang, Gyeonggi, 430-824, Republic of Korea
  • ,
  • Hachung Yoon

      Affiliations

    • OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Institute, National Veterinary Research and Quarantine Service (NVRQS), Anyang, Gyeonggi, 430-824, Republic of Korea
  • ,
  • Young-Ran Heo

      Affiliations

    • Department of Food and Nutrition, Chonnam National University, Yongbongdong, Gwangju, 500-757, Republic of Korea
  • ,
  • Suk-Chan Jung

      Affiliations

    • OIE Reference Laboratory for Brucellosis and Zoonosis Laboratory, Bacteriology and Parasitology Division, Veterinary Research Institute, National Veterinary Research and Quarantine Service (NVRQS), Anyang, Gyeonggi, 430-824, Republic of Korea
  • ,
  • HanSang Yoo

      Affiliations

    • Department of Infectious Diseases, College of Veterinary Medicine, Brain Korea 21 for Veterinary Science, KRF Zoonotic Disease Priority Research Institute, Seoul National University, Gwanak, Seoul, 151-747, Republic of Korea

Received 3 June 2009 ,Accepted 11 December 2009.

References 

  1. Abernethy DA, Pfeiffer DU, Watt R, Denny GO, McCullough S, McDowell SW. Epidemiology of bovine brucellosis in Northern Ireland between 1990 and 2000. Vet. Rec. 2006;158:717–721
  2. Adams LG, Station TAE. NetLibrary I advances in brucellosis research. Texas A&M University, College Station, TX; 1990;
  3. Anderson JD, Smith H. The metabolism of erythritol by Brucella abortus. J. Gen. Microbiol. 1965;38:109–124
  4. Bercovich Z. Maintenance of Brucella abortus-free herds: a review with emphasis on the epidemiology and the problems in diagnosing brucellosis in areas of low prevalence. Vet. Q. 1998;20:81–88
  5. Bricker BJ, Halling SM. Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. J. Clin. Microbiol. 1995;33:1640–1642
  6. Bricker BJ. PCR as a diagnostic tool for brucellosis. Vet. Microbiol. 2002;90:435–446
  7. Burkhardt S, Jimenez de Bagues MP, Liautard JP, Kohler S. Analysis of the behavior of eryC mutants of Brucella suis attenuated in macrophages. Infect. Immun. 2005;73:6782–6790
  8. Clinical Laboratory Standards Institute (CLSI) . Performance standards for antimicrobial susceptability testing; seventeenth informational supplement, M100-S17. Wayne, PA: CLSI; 2007;
  9. Ewalt DR. Comparison of three culture techniques for the isolation of Brucella abortus from bovine supramammary lymph nodes. J. Vet. Diagn. Invest. 1989;1:227–230
  10. Ewalt DR, Bricker BJ. Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51. J. Clin. Microbiol. 2000;38:3085–3086
  11. Farrell ID. The development of a new selective medium for the isolation of Brucella abortus from contaminated sources. Res. Vet. Sci. 1974;16:280–286
  12. Farrell ID, Robertson L. A comparison of various selective media, including a new selective medium for the isolation of Brucellae from milk. J. Appl. Bacteriol. 1972;35:625–630
  13. Foster G, Osterman BS, Godfroid J, Jacques I, Cloeckaert A. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int. J. Syst. Evol. Microbiol. 2007;57:2688–2693
  14. Geering WA, Forman AJ, Nunn MJ, Sciences Australia. Bureau of Resource . Exotic diseases of animals: a field guide for Australian veterinarians. Australian Government Publishing Service, Canberra, Australia; 1995;
  15. Glynn MK, Lynn TV. Brucellosis. J. Am. Vet. Med. Assoc. 2008;233:900–908
  16. Hornsby RL, Jensen AE, Olsen SC, Thoen CO. Selective media for isolation of Brucella abortus strain RB51. Vet. Microbiol. 2000;73:51–60
  17. Hunter D, Kearns M. A comparison of three selective media for the isolation of Brucella abortus from contaminated material. Br. Vet. J. 1977;133:486–489
  18. Jensen AE, Ewalt DR, Cheville NF, Thoen CO, Payeur JB. Determination of stability of Brucella abortus RB51 by use of genomic fingerprint, oxidative metabolism, and colonial morphology and differentiation of strain RB51 from B. abortus isolates from bison and elk. J. Clin. Microbiol. 1996;34:628–633
  19. Jensen AE, Cheville NF, Thoen CO, MacMillan AP, Miller WG. Genomic fingerprinting and development of a dendrogram for Brucella spp. isolated from seals, porpoises, and dolphins. J. Vet. Diagn. Invest. 1999;11:152–157
  20. Jumas-Bilak E, Michaux-Charachon S, Bourg G, Ramuz M, Allardet-Servent A. Unconventional genomic organization in the alpha subgroup of the Proteobacteria. J. Bacteriol. 1998;180:2749–2755
  21. Kattar MM, Zalloua PA, Araj GF, Samaha-Kfoury J, Shbaklo H, Kanj SS, et al. Development and evaluation of real-time polymerase chain reaction assays on whole blood and paraffin-embedded tissues for rapid diagnosis of human brucellosis. Diagn. Microbiol. Infect. Dis. 2007;59:23–32
  22. Mailles A, Garin-Bastuji B, Maurin M, Albert D, Vaillent V (2008). Brucellosis 2008 International Research Conference (including the 61st Brucellosis Research Conference). London: Veterinary Laboratories Agency, pp. 132, 134.
  23. Marin CM, JJiménez de Bagüés MP, Barberán M, Blasco JM. Comparison of two selective media for the isolation of Brucella melitensis from naturally infected sheep and goats. Vet. Rec. 1996;138:409–411
  24. Michaux S, Paillisson J, Carles-Nurit MJ, Bourg G, Allardet-Servent A, Ramuz M. Presence of two independent chromosomes in the Brucella melitensis 16M genome. J. Bacteriol. 1993;175:701–705
  25. Moore CG, Schnurrenberger PR. A review of naturally occurring Brucella abortus infections in wild mammals. J. Am. Vet. Med. Assoc. 1981;179:1105–1112
  26. Moreno E, Stackebrandt E, Dorsch M, Wolters J, Busch M, Mayer H. Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria. J. Bacteriol. 1990;172:3569–3576
  27. Moreno E, Cloeckaert A, Moriyón I. Brucella evolution and taxonomy. Vet. Microbiol. 2002;90:209–227
  28. Nicoletti P. The epidemiology of bovine brucellosis. Adv. Vet. Sci. Comp. Med. 1980;24:69–98
  29. NVRQS . 2006 animal brucellosis international symposium. NVRQS: Anyang; 2006;
  30. In: OIE  editors. Manual of diagnostic tests and vaccines for terrestrial animals. 6th ed.. Paris: Office international des epizootics; 2008;
  31. Paulsen IT, Seshadri R, Nelson KE, Eisen JA, Heidelberg JF, Read TD, et al. The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. Proc. Natl. Acad. Sci. U. S. A. 2002;99:13148–13153
  32. Ragan VE. The Animal and Plant Health Inspection Service (APHIS) brucellosis eradication program in the United States. Vet. Microbiol. 2002;90:11–18
  33. Sangari FJ, García-Lobo JM, Agüero J. The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol. Lett. 1994;121:337–342
  34. Stevens MG, Hennager SG, Olsen SC, Cheville NF. Serologic responses in diagnostic tests for brucellosis in cattle vaccinated with Brucella abortus 19 or RB51. J. Clin. Microbiol. 1994;32:1065–1066
  35. Tessaro SV. The existing and potential importance of brucellosis and tuberculosis in Canadian wildlife: a review. Can. Vet. J. 1986;27:119–124

PII: S0732-8893(09)00504-5

doi: 10.1016/j.diagmicrobio.2009.12.013

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 1 , Pages 15-21 , May 2010