Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 1 , Pages 30-36 , May 2010

Rapid detection of coinfections by Trichomonas vaginalis, Mycoplasma hominis, and Ureaplasma urealyticum by a new multiplex polymerase chain reaction

  • Nicia Diaz

      Affiliations

    • Department of Biomedical Sciences, Gynecology, and Obstetrics, University of Sassari, viale S.Pietro 43/B, 07100, Sassari, Italy
  • ,
  • Daniele Dessì

      Affiliations

    • Department of Biomedical Sciences, Gynecology, and Obstetrics, University of Sassari, viale S.Pietro 43/B, 07100, Sassari, Italy
  • ,
  • Salvatore Dessole

      Affiliations

    • Department of Pharmacology, Gynecology, and Obstetrics, University of Sassari, viale S.Pietro 43/B, 07100, Sassari, Italy
  • ,
  • Pier Luigi Fiori

      Affiliations

    • Department of Biomedical Sciences, Gynecology, and Obstetrics, University of Sassari, viale S.Pietro 43/B, 07100, Sassari, Italy
  • ,
  • Paola Rappelli

      Affiliations

    • Department of Biomedical Sciences, Gynecology, and Obstetrics, University of Sassari, viale S.Pietro 43/B, 07100, Sassari, Italy
    • Corresponding Author InformationCorresponding author. Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, Viale S.Pietro 43/B, 07100 Sassari, Italy. Tel.: +39079228299; fax: +39079212345.

Received 31 July 2009 ,Accepted 22 December 2009.

References 

  1. Abele-Horn M, Wolff C, Dressel P, Zimmermann A, Vahlensieck W, Pfaff F, et al. Polymerase chain reaction versus culture for detection of Ureaplasma urealyticum and Mycoplasma hominis in the urogenital tract of adults and the respiratory tract of newborns. Eur. J. Clin. Microbiol. Infect. Dis. 1996;15:595–598
  2. Adu-Sarkodie Y, Opocu BK, Danso KA, Weiss HA, Mabey D. Comparison of latex agglutination, wet preparation and culture for the detection of Trichomonas vaginalis. Sex Trans. Infect. 2004;80:201–203
  3. Andersen H, Birkelund S, Christiansen G, Freundt EA. Electrophoretic analysis of proteins from Mycoplasma hominis strains detected by SDS-PAGE, two-dimensional gel electrophoresis and immunoblotting. J. Gen. Microbiol. 1987;133:181–191
  4. Black CM, Driebe EM, Howard LA, Fajman NN, Sawyer MK, Girardet RG, et al. Multicenter study of nucleic acid amplification tests for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in children being evaluated for sexual abuse. Pediatr. Infect. Dis. 2009;28:608–613
  5. Blanchard A, Hentschel J, Duffy L, Baldus K, Cassell GH. Detection of Ureaplasma urealyticum by polymerase chain reaction in the urogenital tract of adults, in amniotic fluid, and the respiratory tract of newborns. Clin. Infect. Dis. 1993;17:148–153
  6. Blanchard A, Yaňez A, Dybving K, Watson HL, Griffiths G, Cassel GH. Evaluation of intraspecies genetic variation within the 16S rRNA gene of Mycoplasma hominis and detection by polymerase chain reaction. J. Clin. Microbiol. 1993;31:1358–1361
  7. Dessì D, Delogu G, Emonte E, Catania MR, Fiori PL, Rappelli P. Long-term survival and intracellular replication of Mycoplasma hominis in Trichomonas vaginalis cells: potential role of the protozoon in transmitting bacterial infection. Infect. Immun. 2005;73:1180–1186
  8. Dessì D, Rappelli P, Diaz N, Cappuccinelli P, Fiori PL. Mycoplasma hominis and Trichomonas vaginalis: a unique case of symbiotic relationship between two obligate human parasites. Front. Biosci. 2006;11:2028–2034
  9. Diamond LS. A comparative study of 28 culture media for Trichomonas gallinae. J. Parasitol. 1957;43:488–490
  10. Evans GE, Anderson TP, Seaward LM, Murdoch DR. Evaluation of the Mycoplasma Duo kit for the detection of Mycoplasma hominis and Ureaplasma urealyticum from urogenital and placental specimens. Br. J. Biomed. Sci. 2007;64:66–69
  11. Fang SL, Xiao JC, Lun ZR. Detection of Mycoplasma hominis in Trichomonas vaginalis by PCR. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006;30:144–145
  12. Fichorova RN, Rheinwald JG, Anderson DJ. Generation of papillomavirus-immortalized cell lines from normal human ectocervical, endocervical, and vaginal epithelium that maintain expression of tissue-specific differentiation proteins. Biol. Reprod. 1997;57:847–855
  13. Fortenberry JD, Brizendine EJ, Katz BP, Wools KK, Blythe MJ, Orr DP. Subsequent sexually transmitted infections among adolescent women with genital infection due to Chlamydia trachomatis, Neisseria gonorrhoeae, or Trichomonas vaginalis. Sex Transm. Dis. 1999;26:26–32
  14. Garber GE. The laboratory diagnosis of Trichomonas vaginalis. Can. J. Infect. Dis. Med. Microbiol. 2005;16:35–38
  15. Garcia A, Exposto F, Prieto E, Lopes M, Duarte A, Correia da Silva R. Association of Trichomonas vaginalis with sociodemographic factors and other STDs among female inmates in Lisbon. Int. J. STD AIDS. 2004;15:615–618
  16. Koch A, Bilina A, Teodorowicz L, Stary A. Mycoplasma hominis and Ureaplasma urealyticum in patients with sexually transmitted diseases. Wien. Klin. Wochenschr. 1997;109:584–589
  17. Krieger JN, Milton RT, Stevens CE, Nielsen IO, Hale J, Kiviat NB, et al. Diagnosis of trichomoniasis: comparison of conventional wet-mount examination with cytologic studies, cultures and monoclonal antibody staining of direct specimens. JAMA. 1988;259:1223–1227
  18. Lawing L, Hedges S, Schwebke J. Detection of trichomonosis in vaginal and urine specimens from women by culture and PCR. J. Clin. Microbiol. 2000;38:3585–3588
  19. Madico G, Quinn TC, Rompalo A, McKee KT, Gaydos CA. Diagnosis of Trichomonas vaginalis infection by PCR using vaginal swab samples. J. Clin. Microbiol. 1998;36:3205–3210
  20. Mason PR, Fiori PL, Cappuccinelli P, Rappelli P, Gregson S. Seroepidemiology of Trichomonas vaginalis in rural women in Zimbabwe and patterns of association with HIV infection. Epidemiol. Infect. 2005;133:315–323
  21. McClelland RS, Sangare L, Hassan WM, Lavreys L, Mandaliya K, Kiarie J, et al. Infection with Trichomonas vaginalis increases the risk of HIV-1 acquisition. J. Infect. Dis. 2007;195:698–702
  22. Moss TR, Van Der Pol B. Dual infection with Neisseria gonorrhoeae and Chlamydia trachomatis. Int. J. STD AIDS. 2009;20:143–144
  23. Nassar FA, Abu-Elamreen FH, Shubair ME, Sharif FA. Detection of Chlamydia trachomatis and Mycoplasma hominis, genitalium and Ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria. Adv. Med. Sci. 2008;53:80–86
  24. Ochsendorf FR. Sexually transmitted infections: impact on male fertility. Andrologia. 2008;40:72–75
  25. Pararas MV, Skevaki CL, Kafetzis DA. Preterm birth due to maternal infection: causative pathogens and modes of prevention. Eur. J. Clin. Microbiol. Infect. Dis. 2006;25:562–569
  26. Pastorek JG, Cotch MF, Martin DH, Eschenbach DA. Clinical and microbiological correlates of vaginal trichomoniasis during pregnancy. The Vaginal Infections and Prematurity Study Group. Clin. Infect. Dis. 1996;23:1075–1080
  27. Petrin D, Delgaty K, Bhatt R, Garber G. Clinical and microbiological aspects of Trichomonas vaginalis. Clin. Microbiol. Rev. 1998;11:300–317
  28. Rappelli P, Addis MF, Carta F, Fiori PL. Mycoplasma hominis parasitism of Trichomonas vaginalis. Lancet. 1998;352:1286
  29. Rappelli P, Addis MF, Carta F, Fiori PL. Exploring a novel perspective on pathogenic relationships: response. Trends Microbiol. 1999;7:98
  30. Rappelli P, Carta F, Delogu G, Addis MF, Dessì D, Cappuccinelli P, et al. Mycoplasma hominis and Trichomonas vaginalis symbiosis: multiplicity of infection and transmissibility of M. hominis to human cells. Arch. Microbiol. 2001;175:70–74
  31. Rappelli P, Rocchigiani AM, Erre G, Colombo M, Cappuccinelli P, Fiori PL. Sequence of cDNA coding for a 65 kDa adhesive protein for the specific detection of Trichomonas vaginalis by PCR. FEMS Microbio. Lett. 1995;129:21–26
  32. Riley DE, Roberts MC, Takayama T, Krieger JN. Development of a polymerase chain reaction-based diagnosis of Trichomonas vaginalis. J. Clin. Microbiol. 1992;30:465–472
  33. Riley DE, Samadpour M, Krieger JN. Detection of variable DNA repeats in diverse eukaryotic microorganisms by a single set of polymerase chain reaction primers. J. Clin. Microbiol. 1991;29:2746–2751
  34. Schwebke JR, Burgess D. Trichomoniasis. Clin. Microbiol. Rev. 2004;17:794–803
  35. Sobel JD. What is new in bacterial vaginosis and trichomoniasis?. Infect. Dis. Clin. 2005;19:387–406
  36. Soper D. Trichomoniasis: under control or controlled?. Am. J. Obstet. Gynecol. 2004;190:281–290
  37. Taylor-Robinson D, Furr PM. Genital mycoplasma infections. Wien. Klin. Wochenschr. 1997;109:578–583
  38. van Belkum A, van der Schee C, van der Meijden WI, Verbrugh HA, Sluiters HJ. A clinical study on the association of Trichomonas vaginalis and Mycoplasma hominis infections in women attending a sexually transmitted disease (STD) outpatient clinic. FEMS Immunol. Med. Microbiol. 2001;32:27–32
  39. Van Der Pol B. Trichomonas vaginalis infection: the most prevalent nonviral sexually transmitted infection receives the least public health attention. Clin. Infect. Dis. 2007;44:23–25
  40. Van Der Pol B, Kwok C, Pierre-Louis B, Rinaldi A, Salata RA, Chen PL, et al. Trichomonas vaginalis infection and human immunodeficiency virus acquisition in African women. J. Infect. Dis. 2008;197:548–554
  41. Wendel KA, Workowski KA. Trichomoniasis: challenges to appropriate management. Clin. Infect. Dis. 2007;44:S123–S129
  42. Zhang ZF, Begg CB. Is Trichomonas vaginalis a cause of cervical neoplasia? Results from a combined analysis of 24 studies. Int. J. Epidemiol. 1994;23:682–690

PII: S0732-8893(09)00513-6

doi: 10.1016/j.diagmicrobio.2009.12.022

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 1 , Pages 30-36 , May 2010