Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 2 , Pages 122-128 , June 2010

Rapid and simultaneous detection of Ureaplasma parvum and Chlamydia trachomatis antibodies based on visual protein microarray using gold nanoparticles and silver enhancement

  • Jingfeng Tang

      Affiliations

    • State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China
    • ,
  • Zhenghui Xu

      Affiliations

    • State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China
    • ,
  • Li Zhou

      Affiliations

    • State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China
    • ,
  • Hong Qin

      Affiliations

    • Hubei Entry–Exit Inspection and Quarantine Bureau, Hubei 430022, China
    • ,
  • Yefu Wang

      Affiliations

    • State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China
    • Corresponding Author InformationCorresponding author. Tel.: +86-27-6875-4627; fax: +86-27-6875-4592.
    • ,
  • Hanhua Wang

      Affiliations

    • Hubei Entry–Exit Inspection and Quarantine Bureau, Hubei 430022, China

Received 24 September 2009 ,Accepted 15 January 2010.

References 

  1. Alexandre I, Hamels S, Dufour S, Collet J, Zammatteo N, De Longueville F, et al. Colorimetric silver detection of DNA microarrays. Anal. Biochem. 2001;295:1–8
  2. Anttila T, Saikku P, Koskela P, et al. Serotypes of Chlamydia trachomatis and risk for development of cervical squamous cell carcinoma. JAMA. 2001;285:47–51
  3. Bowtelld DL. Options available from start to finish—for obtaining expression data by microarray. Nat. Genet. 1999;21:25–32
  4. Brunham RC, Rey LJ. Immunology of Chlamydia infection: implications for a Chlamydia trachomatis vaccine. Nat. Rev. Immunol. 2005;5:149–161
  5. Brunham RC, Zhang DJ, Yang X, et al. The potential for vaccine development against chlamydial infection and disease. J. Infect. Dis. 2000;181:538
  6. Cao X, Wang YF, Zhang CF. Visual DNA microarrays for simultaneous detection of Ureaplasma urealyticum and Chlamydia trachomatis coupled with multiplex asymmetrical PCR. Biosens. Bioelectron. 2006;22:393–398
  7. Cao X, Jiang ZY, Wang YF, Gong R, Zhang CF. Two multiplex real-time TaqMan polymerase chain reaction systems for simultaneous detecting and serotyping of Ureaplasma parvum. Diagn Micr Infec Dis. 2007;59:109–111
  8. Duan LL, Wang YF, Li SC, Wan ZX, Zhai JX. Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method. BMC Infect. Dis. 2005;5:53
  9. Haab BB, Dunham MJ, Brown PO. Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions. Genome Biol. 2001;2:1–13
  10. Heather EF, Heather MC, Richard HA. Surface expression, single-channel analysis and membrane topology of recombinant Chlamydia trachomatis major outer membrane protein. BMC Microbiol. 2005;53:1–8
  11. Kong F, Zhu X, Wang W, et al. Comparative analysis and serovar-specific identification of multiple-banded antigen genes of Ureaplasma urealyticum biovar 1. J. Clin. Microbiol. 1999;37:538–543
  12. Li ZY, Wu YM, Chen CQ, Wan YP, Zhu CM. Expression and purification of the major outer membrane protein of Chlamydia trachomatis in prokaryotic cell. J. Microbiol. Immunol. 2004;2:62–65
  13. Liu HHi, Cao X, Yang Y, Liu MG, Wang YF (2006) Array-based nano-amplification technique was applied in detection of hepatitis E virus. J Biochem Mol Biol 39:5, 247–252.
  14. Mezzasoma L, Bacarese HT, Di CM, Rossi R, Bistoni F, Crisanti A. Antigen microarrays for serodiagnosis of infectious diseases. Clin. Chem. 2002;48:121–130
  15. Morrisn RP, Caldwell HD. Immunity to murine chlamydial genital infection. Infect. Immun. 2002;70:2741–2751
  16. Patrick D, Ursula S, Johanna P, Claudia P. Protein microarray for the analysis of human melanoma biomarkers. Sensor. Actuat. 2009;B 139:2–8
  17. Pragya S, Rishein G, Hem CJ, Rajneesh J, Apurb RB, Sudha S, et al. Serovar-specific immune responses to peptides of variable regions of Chlamydia trachomatis major outer membrane protein in serovar D-infected women. Clin. Exp. Med. 2008;8:207–215
  18. Robertson JA, Stemke GW, Davis JJW, et al. Proposal of Ureaplasma parvum sp. nov. and emended description of Ureaplasma urealyticum. Int. J. Syst. Evol. Microbiol. 2002;52:587–597
  19. Schena M, Renu A. Microarrays: biotechnology's discovery platform for functional genomics. Tib, Tech. 1998;16:301–306
  20. Shree RS, Kara H, Shreekumar RP, Vida AD, MK O, Karyn SG. Mucosal immunization with recombinant MOMP genetically linked with modified cholera toxin confers protection against Chlamydia trachomatis infection. Vaccine. 2006;24:1213–1224
  21. Tang JF, Zhou L, Gao WJ, Cao X, Wang YF. Visual DNA microarrays for simultaneous detection of Human immunodeficiency virus type-1 and Treponema pallidum coupled with multiplex asymmetrical PCR. Diagn. Microbiol. Infect. Dis. 2009;65:372–378
  22. Taton TA, Mirkin CA, Letsinger RL. Scanometric DNA array detection with nanoparticle probes. Science. 2000;289:1757–1760
  23. Teng LJ, Zheng X, Glass JI, Watson HL, Tsai J, Cassell GH. Ureaplasma urealyticum biovar specificity and diversity are encoded in multiple-banded antigen gene. J. Clin. Microbiol. 1994;32:1464–1469
  24. Tyers M, Mann M. From genomics to proteomics. Nature. 2003;422:193–197
  25. Wang YF, Pang DW, Zhang ZL, Zheng HZ, Cao JP, Shen JT. Visual gene diagnosis of HBV and HCV based on nanoparticle probe amplification and silver staining enhancement. J. Med. Virol. 2003;70:205–211
  26. Wang YF, Gao WJ, Wang HQ, Luo YH. Preparation of a visual protein chip for detection of IgG against Treponema pallidum. Mol. Biotech. 2005;31:121–128
  27. Wang ZX, Jason L, Andrew R, Cossins , Mathias B. Microarray-based detection of protein binding and functionality by gold nanoparticle probes. Anal. Chem. 2005;77:5770–5774
  28. Zhang YQ, Wang YF, Jiang XD, et al. The application of nano-particles in biochip. Rec. Pat. Biotech. 2008;2:55–59
  29. Zhu H, Snyder M. Protein arrays and microarrays. Curr. Opin. Chem. Biol. 2001;5:40–45
  30. Zhu H, Snyder M. Protein chip technology. Curr. Opin. Chem. Biol. 2003;7:55–63

PII: S0732-8893(10)00013-1

doi: 10.1016/j.diagmicrobio.2010.01.009

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 2 , Pages 122-128 , June 2010

Article Tools

* Image Usage & Resolution