Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 2 , Pages 134-142 , June 2010

Design and validation of a multiplex polymerase chain reaction for the identification of enterotoxigenic Escherichia coli and associated colonization factor antigens

  • Rania A. Nada
    • ,
  • Hind I. Shaheen
    • ,
  • Iman Touni
    • ,
  • Dina Fahmy

      Affiliations

    • Present address: Dina Fahmy: Royal Melbourne Institute of Technology, Melbourne VIC 3001, Australia.
    • ,
  • Adam W. Armstrong

      Affiliations

    • Present address: Adam W. Armstrong: National Naval Medical Center, US Navy Central HIV Program, 8901 Rockville Pike, Bethesda, MD 20889-5600.
    • ,
  • Matthew Weiner

      Affiliations

    • Present address: Matthew Weiner: Biological Defense Research Directorate, US Naval Medical Research Center, Silver Spring, MD 20910.
    • ,
  • John D. Klena

      Affiliations

    • Corresponding Author InformationCorresponding author. Tel.: +202-2348-0349; fax: +202-2342-3428.

Received 20 October 2009 ,Accepted 31 January 2010.

References 

  1. Anantha RP, McVeigh AL, Lee LH, Agnew MK, Cassels FJ, Scott DA, et al. Evolutionary and functional relationships of colonization factor antigen i and other class 5 adhesive fimbriae of enterotoxigenic Escherichia coli. Infect. Immun. 2004;72:7190–7201
  2. Binsztein N, Jouve MJ, Viboud GI, Lopez Moral L, Rivas M, Orskov I, et al. Colonization factors of enterotoxigenic Escherichia coli isolated from children with diarrhea in Argentina. J. Clin. Microbiol. 1991;29:1893–1898
  3. Bolin I, Wiklund G, Qadri F, Torres O, Bourgeois AL, Savarino S, et al. Enterotoxigenic Escherichia coli with STh and STp genotypes is associated with diarrhea both in children in areas of endemicity and in travelers. J. Clin. Microbiol. 2006;44:3872–3877
  4. Cassels FJ, Wolf MK. Colonization factors of diarrheagenic E. coli and their intestinal receptors. J. Ind. Microbiol. 1995;15:214–226
  5. Gaastra W, Svennerholm AM. Colonization factors of human enterotoxigenic Escherichia coli (ETEC). Trends Microbiol. 1996;4:444–452
  6. Giron JA, Viboud GI, Sperandio V, Gomez-Duarte OG, Maneval DR, Albert MJ, et al. Prevalence and association of the longus pilus structural gene (lngA) with colonization factor antigens, enterotoxin types, and serotypes of enterotoxigenic Escherichia coli. Infect. Immun. 1995;63:4195–4198
  7. Giron JA, Gomez-Duarte OG, Jarvis KG, Kaper JB. Longus pilus of enterotoxigenic Escherichia coli and its relatedness to other type-4 pili—a minireview. Gene. 1997;192:39–43
  8. Grewal HM, Valvatne H, Bhan MK, van Dijk L, Gaastra W, Sommerfelt H. A new putative fimbrial colonization factor, CS19, of human enterotoxigenic Escherichia coli.. Infect. Immun. 1997;65:507–513
  9. Grewal HM, Sommerfelt H. Genotypic detection of enterotoxigenic Escherichia coli colonisation factors. APMIS. 2001;109:447–453
  10. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. http://www.mbio.ncsu.edu/BioEdit/bioedit.html. Nucleic Acids Symp Ser 41:95–98.
  11. Klena JD, Parker CT, Knibb K, Ibbitt JC, Devane PML, Horn ST, et al. Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA.. J. Clin. Microbiol. 2004;42(12):5549–5557
  12. Liu PY, Lau YJ, Hu BS, Shyr JM, Shi ZY, Tsai WS, et al. Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods. J. Clin. Microbiol. 1995;33:1779–1783
  13. Lopez-Vidal Y, Svennerholm AM. Monoclonal antibodies against the different subcomponents of colonization factor antigen II of enterotoxigenic Escherichia coli. J. Clin. Microbiol. 1990;28:1906–1912
  14. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 1998;11:142–201
  15. Nicklasson M, Sjoling A, Lebens M, Tobias J, Janzon A, Brive L, et al. Mutations in the periplasmic chaperone leading to loss of surface expression of the colonization factor CS6 in enterotoxigenic Escherichia coli (ETEC) clinical isolates. Microb. Pathog. 2008;44:246–254
  16. Peruski LF, Kay BA, El-Yazeed RA, El-Etr SH, Cravioto A, Wierzba TF, et al. Phenotypic diversity of enterotoxigenic Escherichia coli strains from a community-based study of pediatric diarrhea in periurban Egypt. J. Clin. Microbiol. 1999;37:2974–2978
  17. Pichel M, Binsztein N, Viboud G. CS22, a novel human enterotoxigenic Escherichia coli adhesin, is related to CS15. Infect. Immun. 2000;68:3280–3285
  18. Qadri F, Khan AI, Faruque AS, Begum YA, Chowdhury F, Nair GB, et al. Enterotoxigenic Escherichia coli and Vibrio cholerae diarrhea, Bangladesh, 2004.. Emerg. Infect. Dis. 2005;11:1104–1107
  19. Rao MR, Abu-Elyazeed R, Savarino SJ, Naficy AB, Wierzba TF, Abdel-Messih I, et al. High disease burden of diarrhea due to enterotoxigenic Escherichia coli among rural Egyptian infants and young children. J. Clin. Microbiol. 2003;41:4862–4864
  20. Reischl U, Youssef MT, Wolf H, Hyytia-Trees E, Strockbine NA. Real-time fluorescence PCR assays for detection and characterization of heat-labile I and heat-stable I enterotoxin genes from enterotoxigenic Escherichia coli. J. Clin. Microbiol. 2004;42:4092–4100
  21. Rockabrand DM, Shaheen HI, Khalil SB, Peruski LF, Rozmajzl PJ, Savarino SJ, et al. Enterotoxigenic Escherichia coli colonization factor types collected from 1997 to 2001 in US military personnel during operation Bright Star in northern Egypt. Diagn. Microbiol. Infect. Dis. 2006;55:9–12
  22. Rodas C, Iniguez V, Qadri F, Wiklund G, Svennerholm AM, Sjoling A. Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins. J. Clin. Microbiol. 2009;47:1218–1220
  23. Sanchez J, Holmgren J, Svennerholm AM. Recombinant fusion protein for simple detection of Escherichia coli heat-stable enterotoxin by GM1 enzyme-linked immunosorbent assay. J. Clin. Microbiol. 1990;28:2175–2177
  24. Shaheen HI, Khalil SB, Rao MR, Abu Elyazeed R, Wierzba TF, Peruski LF, et al. Phenotypic profiles of enterotoxigenic Escherichia coli associated with early childhood diarrhea in rural Egypt. J. Clin. Microbiol. 2004;42:5588–5595
  25. Shaheen HI, Abdel Messih IA, Klena JD, Mansour A, El-Wakkeel Z, Wierzba TF, et al. Phenotypic and genotypic analysis of enterotoxigenic Escherichia coli in samples obtained from Egyptian children presenting to referral hospitals. J. Clin. Microbiol. 2009;47:189–197
  26. Sizemore DR, Roland KL, Ryan US. Enterotoxigenic Escherichia coli virulence factors and vaccine approaches. Expert Rev. Vaccines. 2004;3:585–595
  27. Sjoling A, Wiklund G, Savarino SJ, Cohen DI, Svennerholm AM. Comparative analyses of phenotypic and genotypic methods for detection of enterotoxigenic Escherichia coli toxins and colonization factors. J. Clin. Microbiol. 2007;45:3295–3301
  28. Stacy-Phipps S, Mecca JJ, Weiss JB. Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection. J. Clin. Microbiol. 1995;33:1054–1059
  29. Steinsland H, Valentiner-Branth P, Grewal HM, Gaastra W, Molbak KK, Sommerfelt H. Development and evaluation of genotypic assays for the detection and characterization of enterotoxigenic Escherichia coli. Diagn. Microbiol. Infect. Dis. 2003;45:97–105
  30. Svennerholm AM, Wiklund G. Rapid GM1-enzyme–linked immunosorbent assay with visual reading for identification of Escherichia coli heat-labile enterotoxin. J. Clin. Microbiol. 1983;17:596–600
  31. Svennerholm AM, Steele D. Microbial-gut interactions in health and disease. Progress in enteric vaccine development.. Best Pract. Res. Clin. Gastroenterol. 2004;18:421–445
  32. Svennerholm AM, Tobias J. Vaccines against enterotoxigenic Escherichia coli. Expert Rev. Vaccines. 2008;7:795–804
  33. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007;24:1596–1599
  34. Tobias J, Lebens M, Kallgard S, Nicklasson M, Svennerholm AM. Role of different genes in the CS6 operon for surface expression of enterotoxigenic Escherichia coli colonization factor CS6. Vaccine. 2008;26:5373–5380
  35. Wenneras C, Erling V. Prevalence of enterotoxigenic Escherichia coli-associated diarrhoea and carrier state in the developing world. J. Health Popul. Nutr. 2004;22:370–382
  36. Wierzba TF, Abdel-Messih IA, Abu-Elyazeed R, Putnam SD, Kamal KA, Rozmajzl P, et al. Clinic-based surveillance for bacterial- and rotavirus-associated diarrhea in Egyptian children.. Am. J. Trop. Med. Hyg. 2006;74(1):148–153
  37. Wolf MK. Occurrence, distribution, and associations of O and H serogroups, colonization factor antigens, and toxins of enterotoxigenic Escherichia coli. Clin. Microbiol. Rev. 1997;10:569–584

PII: S0732-8893(10)00035-0

doi: 10.1016/j.diagmicrobio.2010.01.011

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 2 , Pages 134-142 , June 2010

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