Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 2 , Pages 153-161, June 2010

Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasis☆

  • Nágilla Daliane Feliciano

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
    • ,
  • Henrique Tomaz Gonzaga

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
    • ,
  • Maria do Rosário Fátima Gonçalves-Pires

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
    • ,
  • Ana Lúcia Ribeiro Gonçalves

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
    • ,
  • Rosangela Maria Rodrigues

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
    • ,
  • Marlene Tiduko Ueta

      Affiliations

    • Department of Parasitology, Institute of Biology, State University of Campinas, 13083-970, Campinas, SP, Brazil
    • ,
  • Julia Maria Costa-Cruz

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
    • Corresponding Author InformationCorresponding author. Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Laboratório de Diagnóstico de Parasitoses, Universidade Federal de Uberlândia, Avenida Pará 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brasil. Tel.: +55-34-32182187; fax: +55-34-31182332.

Received 23 November 2009; accepted 31 January 2010. published online 01 April 2010.

Abstract 

The objective of the present research was to evaluate detergent and aqueous phases of total saline (TS) and alkaline extracts of Strongyloides venezuelensis for human strongyloidiasis immunodiagnosis. Total extracts and detergent and aqueous antigenic fractions were separated using Triton X-114 and were examined by enzyme-linked immunosorbent assay (ELISA) and immunoblotting (IB) tests to detect immunoglobulin G (IgG). Serum samples were obtained from 120 individuals: 40 strongyloidiasis patients (group I), 40 patients with other parasitic diseases (group II), and 40 apparently healthy individuals (group III). Each extract provided a different profile of antigenic components as recognized by IgG in IB. The detergent fraction of the TS extract demonstrated the highest sensitivity and specificity for ELISA and IB. The results indicated that the detergent saline fraction, purified from S. venezuelensis, furnished the most valid results for the strongyloidiasis immunodiagnosis and could be employed as an alternative antigen and as a useful source of specific polypeptides.

Keywords: Hydrophobic fractions, Immunodiagnosis, Strongyloides venezuelensis, Strongyloidiasis

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☆ This research was supported by the Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and by the Federal University of Uberlândia, Brazil.

PII: S0732-8893(10)00036-2

doi:10.1016/j.diagmicrobio.2010.01.012

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 2 , Pages 153-161, June 2010

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