Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 2 , Pages 153-161 , June 2010

Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasis

  • Nágilla Daliane Feliciano

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
  • ,
  • Henrique Tomaz Gonzaga

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
  • ,
  • Maria do Rosário Fátima Gonçalves-Pires

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
  • ,
  • Ana Lúcia Ribeiro Gonçalves

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
  • ,
  • Rosangela Maria Rodrigues

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
  • ,
  • Marlene Tiduko Ueta

      Affiliations

    • Department of Parasitology, Institute of Biology, State University of Campinas, 13083-970, Campinas, SP, Brazil
  • ,
  • Julia Maria Costa-Cruz

      Affiliations

    • Department of Immunology, Microbiology and Parasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
    • Corresponding Author InformationCorresponding author. Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Laboratório de Diagnóstico de Parasitoses, Universidade Federal de Uberlândia, Avenida Pará 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brasil. Tel.: +55-34-32182187; fax: +55-34-31182332.

Received 23 November 2009 ,Accepted 31 January 2010.

References 

  1. Atkins NS, Conway DJ, Lindo JF, Bailey JW, Bundy DPA. L3 antigen-specific antibody isotype responses in human strongyloidiasis: correlations with larval output. Parasite Immunol. 1999;21:517–526
  2. Baermann G. Eine Einfache Methode zur Auffinfung von Ankylostomun (Nematoden) Larven in Erdproben. Mededeel. mith H Geneesk. Batavia Lab Weltevreden Feestbundel. 1917;41–47
  3. Blat JM, Cantos A. Evaluation of techniques for the diagnosis of Strongyloides stercoralis in human immunodeficiency virus (HIV) positive and HIV negative individuals in the city of Itajaí, Brazil. J. Infect. Dis. 2003;7:402–408
  4. Bordier C. Phase separation of integral membrane proteins in Triton-114 solution. J. Biol. Chem. 1981;256:1604–1607
  5. Bricker TM, Prevost M, Vu V, Laborde S, Womack J, Frankel LK. Isolation of luminal proteins from spinach thylakoid membranes by Triton X-114 phase portioning. Biochim. Biophys. Acta. 2001;1503:350–356
  6. Chaturvedi V, Gupta HP. Evaluation of integral membrane antigens of Mycobacterium habana for serodiagnosis of extrapulmonary tuberculosis: association between levels of antibodies and Mycobacterium tuberculosis antigens. FEMS Immunol. Med. Microbiol. 2002;33:1–7
  7. Conway DJ, Bailey JW, Lindo JF, Robinson RD, Bundy DA, Bianco AE. Serum IgG reactivity with 41-, 31-, and 28-kDa larval proteins of Strongyloides stercoralis in individuals with strongyloidiasis. J. Infect. Dis. 1993;168:784–787
  8. Conway DJ, Lindo JF, Robinson RD, Bundy DA, Bianco AE. Strongyloides stercoralis: characterization of immunodiagnostic larval antigens. Exp. Parasitol. 1994;79:99–105
  9. Costa-Cruz JM, Bullamah CB, Gonçalves-Pires MRF, Campos DM, Vieira MA. Cryo-microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis. Rev. Inst. Med. Trop. São Paulo. 1997;39:313–317
  10. Costa-Cruz JM, Bullamah CB, Gonçalves-Pires MRF, Campos DMB. Soroepidemiological study of human strongyloidiasis with blood samples collected on filter paper, in Abadia dos Dourados (Minas Gerais, Brazil). Rev. Inst. Med. Trop. São Paulo. 1998;40:329–331
  11. Dreyer G, Fernandes-Silva E, Alves S, Rocha A, Albuquerque R, Addiss D. Patterns of detection of Strongyloides stercoralis in stool specimens: implications for diagnosis and clinical trials. J. Clin. Microbiol. 1996;34:2569–2571
  12. Ferreira MS, Nishioka SA, Borges AS, Costa-Cruz JM, Rossin IR, Rocha A, et al. Strongyloidiasis and infection due to human immunodeficiency virus: 25 cases at a Brazilian Teaching Hospital, including seven cases of hyperinfection syndrome. Clin. Infect. Dis. 1999;28:154–155
  13. Greiner M, Sohr D, Gobel P. A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests. J. Immunol. Methods. 1995;185:123–132
  14. Grove DI, Blair AJ. Diagnosis of human strongyloidiasis by immunofluorescence, using Strongyloides ratti and Strongyloides stercoralis larvae. Am. J. Trop. Med. Hyg. 1981;30:344–349
  15. Grove DI. Human strongyloidiasis. Adv. Parasitol. 1996;38:251–309
  16. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;277:680–685
  17. Lindo JF, Conway DJ, Atkins NS, Bianco AE, Robinson RD, Bundy DAP. Prospective evaluation of enzyme-linked immunosorbent assay and immunoblot methods for the diagnosis of endemic Strongyloides stercoralis infection. Am. J. Trop. Med. Hyg. 1994;51:175–179
  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951;29:265–275
  19. Lutz A. O Schistosomun mansoni e a schistosomatose segundo observações feitas no Brasil. Mem. Inst. Oswaldo Cruz. 1919;11:121–155
  20. Machado ER, Ueta MT, Gonçalves-Pires MRF, Alves de Oliveira JBA, Faccioli LH, Costa-Cruz JM. Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay. Mem. Inst. Oswaldo Cruz. 2003;98:849–851
  21. Machado ER, Ueta MT, Gonçalves-Pires MRF, de Oliveira JBA, Faccioli LH, Costa-Cruz JM. Diagnosis of human strongyloidiasis using particulate antigen of two strains of Strongyloides venezuelensis in indirect immunofluorescence antibody test. Exp. Parasitol. 2001;99:52–55
  22. Machado GA, Santiago FM, Mineo JR, Costa-Cruz JM. Assessment of antigenic fractions of varying hydrophobicity from Taenia solium metacestodes for the diagnosis of human neurocysticercosis. Trop. Med. Int. Health. 2007;12:1369–1376
  23. Mota-Ferreira DML, Gonçalves-Pires MRF, Ferreira-Júnior A, Sopelete MC, Abdallah VOS, Costa-Cruz JM. Specific IgA and IgG antibodies in paired serum and Bread Milk samples in human strongyloidiasis. Act. Trop. 2009;109:103–107
  24. Oliveira LCM, Ribeiro CT, Mendes DM, Oliveira TC, Costa Cruz JM. Frequency of Strongyloides stercoralis infection in alcoholics. Mem. Inst. Oswaldo Cruz. 2002;97:119–121
  25. Rodrigues RM, Sopelete MC, Silva DAO, Cunha-Junior JP, Taketomi EA, Costa-Cruz JM. Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis. Mem. Inst. Oswaldo Cruz. 2004;99:89–93
  26. Rossi CL, Takahashi EE, Teixeira AL, Barros-Mazon S, Trevisan RC. Avaliação de preparações antigênicas de Strongyloides stercoralis para o imunodiagnóstico da estrongiloidíase. Rev. Soc. Bras. Med. Trop. 1993;26:83–87
  27. Roxby AC, Gottlieb GS, Limaye AP. Strongyloidiasis in transplant patients. Clin. Infect. Dis. 2009;49:1411–1423
  28. Rugai E, Matton T, Brisola AP. Nova técnica para isolar larvas de nematóides das fezes: modificação do método de Baermann. Rev. Inst. Adolfo Lutz. 1954;14:5–8
  29. Sato Y, Inque F, Matsuyama R, Shiroma Y. Immunoblot analysis of antibodies in human strongyloidiasis. Trans. R. Soc. Trop. Med. Hyg. 1990;84:403–406
  30. Sato Y, Kobayashi J, Toma H, Shiroma Y. Efficacy of stool examination for detection of Strongyloides infection. Am. J. Trop. Med. Hyg. 1995;53:248–250
  31. Siddiqui AA, Berk SL. Diagnosis of Strongyloides stercoralis infection. Clin. Infect. Dis. 2001;33:1040–1047
  32. Silva LP, Barcelos IS, Passos-Lima AB, Espindola FS, Campos DM, Costa-Cruz JM. Western Blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis. Mem. Inst. Oswaldo Cruz. 2003;98:687–689
  33. Sithithaworn P, Srisawangwong T, Tesana S, Daenseekaew W, Sithithaworn J, Fujimaki Y, et al. Epidemiology of Strongyloides stercoralis in north-east Thailand: application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay. Trans. R. Soc. Trop. Med. Hyg. 2003;97:398–402
  34. Sopelete MC. Métodos de análise em estudos sobre diagnóstico. In:  Mineo JR,  Silva DAO,  Sopelete MC,  Leal GS,  Vidigal LHG,  Tápia LHR, et al. editor. Pesquisa na área biomédica: do planejamento à publicação. 2005;p. 203–214Uberlândia, Minas Gerais, Brazil
  35. Sudré AP, Siqueira RC, Barreto MGM, Peralta RHS, Macedo HM, Peralta JM. Identification of a 26 kDa protein fraction as an important antigen for application in the immunodiagnosis of strongyloidiasis. Parasitol. Res. 2007;101:1117–1123
  36. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc. Natl. Acad. Sci. U. S. A. 1979;76:4350–4354
  37. Uparanukraw P, Phongsri S, Morakote N. Fluctuations of larval excretion in Strongyloides stercoralis infection. Am. J. Trop. Med. Hyg. 1999;60:967–973
  38. Vadlamudi RS, Chi DS, Krishnaswamy G. Intestinal strongyloidiasis and hyperinfection syndrome. Clin. Mol. Allergy. 2006;30:4–8

 This research was supported by the Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and by the Federal University of Uberlândia, Brazil.

PII: S0732-8893(10)00036-2

doi: 10.1016/j.diagmicrobio.2010.01.012

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 2 , Pages 153-161 , June 2010