Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 3 , Pages 239-245, July 2010

Evaluation of a real-time polymerase chain reaction assay for the detection of Dientamoeba fragilis☆

  • Adriana Calderaro

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39-0521-988885; fax: +39-0521-993620.
    • ,
  • Chiara Gorrini

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
    • ,
  • Sara Montecchini

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
    • ,
  • Simona Peruzzi

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
    • ,
  • Giovanna Piccolo

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
    • ,
  • Sabina Rossi

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
    • ,
  • Franco Gargiulo

      Affiliations

    • Department of Experimental and Applied Medicine, Institute of Microbiology, Spedali Civili, University of Brescia, 25123 Brescia, Italy
    • ,
  • Nino Manca

      Affiliations

    • Department of Experimental and Applied Medicine, Institute of Microbiology, Spedali Civili, University of Brescia, 25123 Brescia, Italy
    • ,
  • Giuseppe Dettori

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy
    • ,
  • Carlo Chezzi

      Affiliations

    • Department of Pathology and Laboratory Medicine, Section of Microbiology, University Hospital of Parma, 43100 Parma, Italy

Received 9 December 2009; accepted 14 February 2010. published online 12 May 2010.

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☆ A.C. and C.G. conceived and designed the experiments. A.C., C.G., S.M., S.P., and G.P. performed the experiments. A.C. and S.R. performed conventional parasitological diagnostic methods. S.M., F.G., and N.M. performed sequencing. C.G. and S.M. did the sequence analysis. A.C., C.G., and S.M. wrote the paper. G.D. and C.C. conducted scientific supervision of the manuscript.

PII: S0732-8893(10)00052-0

doi:10.1016/j.diagmicrobio.2010.02.016

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 3 , Pages 239-245, July 2010

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