Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 3 , Pages 213-219 , July 2010

Detection of Helicobacter pylori and clarithromycin resistance in gastric biopsies of pediatric patients by using a commercially available real-time polymerase chain reaction after NucliSens semiautomated DNA extraction

  • Sonia Agudo

      Affiliations

    • Department of Microbiology, Hospital De La Princesa, 28006 Madrid, Spain
    • Corresponding Author InformationCorresponding author. Tel.: +34-915202317; fax: +34-915202317.
    • ,
  • Teresa Alarcón

      Affiliations

    • Department of Microbiology, Hospital De La Princesa, 28006 Madrid, Spain
    • ,
  • Pedro Urruzuno

      Affiliations

    • Department of Gastroenterology, Hospital Doce Octubre, 28041 Madrid, Spain
    • ,
  • María Josefa Martínez

      Affiliations

    • Department of Gastroenterology, Hospital Niño Jesus, 28009 Madrid, Spain
    • ,
  • Manuel López-Brea

      Affiliations

    • Department of Microbiology, Hospital De La Princesa, 28006 Madrid, Spain

Received 10 November 2009 ,Accepted 26 February 2010.

References 

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  2. Burucoa C, Garnier M, Silvain C, Fauchere JL. Quadruplex real-time PCR assay using allele-specific scorpion primers for detection of mutations conferring clarithromycin resistance to Helicobacter pylori. J. Clin. Microbiol. 2008;46:2320–2326
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  10. López-Brea M, Alarcón T. Novel real time PCR for detection of clarithromycin resistance in Helicobacter pylori clinical isolates. Helicobacter. 2006;11:400
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  16. Osato M, Reddy R, Reddy SG, Penland R, Malaty H, Graham D. Pattern of primary resistance of Helicobacter pylori to metronidazole or clarithromycin in the United States. Arch. Intern. Med. 2001;161:1217–1220
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  22. Van Doorn L, Glupczynski Y, Kusters J, Megraud F, Midolo P, Maggi-Solca N, et al. Accurate prediction of macrolide resistance in Helicobacter pylori by a PCR line probe assay for detection of mutations in the 23S rRNA gene: multicenter validation study. Antimicrob. Agents Chemother. 2001;45:1500–1504
  23. Versalovic J. Helicobacter pylori. Pathology and diagnostic strategies. Am. J. Clin. Pathol. 2003;119:403–412

☆ This work was supported by Fondo de Investigación Sanitarias de la Seguridad Social, grant FIS PI 052452.

PII: S0732-8893(10)00077-5

doi: 10.1016/j.diagmicrobio.2010.02.021

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 3 , Pages 213-219 , July 2010

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