Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 3 , Pages 234-238 , July 2010

Identification of acid-fast bacilli using pyrosequencing analysis

  • Jian R. Bao

      Affiliations

    • Department of Microbiology, Quest Diagnostics Nichols Institute, Chantilly, VA 20151, USA
    • ,
  • Ronald N. Master

      Affiliations

    • Department of Microbiology, Quest Diagnostics Nichols Institute, Chantilly, VA 20151, USA
    • ,
  • Dale A. Schwab

      Affiliations

    • Department of Microbiology, Quest Diagnostics Nichols Institute, San Juan Capistrano, CA 92675, USA
    • ,
  • Richard B. Clark

      Affiliations

    • Department of Microbiology, Quest Diagnostics Nichols Institute, Chantilly, VA 20151, USA
    • Corresponding Author InformationCorresponding author. Department of Microbiology/Virology, Quest Diagnostics Nichols Institute Chantilly, VA 20151, USA.

Received 28 September 2009 ,Accepted 26 February 2010.

References 

  1. Brown BA, Wallace RJ. Mycobacterium: clinical and laboratory characteristics of rapidly growing mycobacteria. In: Murray PR, et al. editor. Manual of Clinical Microbiology. 9th ed.. Washington, DC: ASM Press; 2007;p. 589–600
  2. Brown-Elliott BA, Brown JM, Conville PS, Wallace RJ. Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy. Clin. Microbiol. Rev. 2006;19:259–282
  3. Cloud JL, Neal J, Rosenberry R, Turenne CY, Jama M, Hillyard DR, et al. Identification of Mycobacterium spp. using a commercial 16S ribosomal DNA sequencing kit and additional sequencing libraries. J. Clin. Microbiol. 2002;40:400–406
  4. Cloud JL, Conville PS, Croft A, Harmsen D, Witebsky FG, Carroll KC. Evaluation of partial 16S ribosomal DNA sequencing for identification of Nocardia species by using the MicroSeq 500 system with an expanded database. J. Clin. Microbiol. 2004;42:578–584
  5. Cloud JL, Hoggan K, Belousov E, Cohen S, Brown-Elliott BA, Mann L, et al. Use of the MGB Eclipse System and SmartCycler PCR for differentiation of Mycobacterium chelonae and M. abscessus. J. Clin. Microbiol. 2005;43:4205–4207
  6. Conville PS, Witebsky FG. Nocardia, Rhodococcus, Gordonia, Actinomadura, Streptomyces, and other aerobic actinomycetes. In: Murray PR, et al. editor. Manual of Clinical Microbiology. 9th ed.. Washington, DC: ASM Press; 2007;p. 515–542
  7. Conville PS, Zelazny AM, Witebsky FG. Analysis of secA1 gene sequences for identification of Nocardia species. J. Clin. Microbiol. 2006;44:2760–2766
  8. Dei R, Tortoli E, Bartoloni A, Simonetti MT, Lillini E. HPLC does not differentiate Mycobacterium paratuberculosis from Mycobacterium avium. Vet. Microbiol. 1999;65:209–213
  9. Galor A, Hall GS, Procop GW, Tuohy M, Millstein ME, Jeng BH. Rapid species determination of Nocardia keratitis using pyrosequencing technology. Am. J. Ophthalmol. 2007;143:182–183
  10. Heller LC, Jones M, Widen RH. Comparison of DNA pyrosequencing with alternative methods for identification of mycobacteria. J. Clin. Microbiol. 2008;46:2092–2094
  11. Iannotti CS, Hall GS, Procop GW, Tuohy MJ, Staugaitis SM, Wiel RJ. Solitary Nocardia farcinica brain abscess in an immunocompetent adult mimicking metastatic brain tumor: rapid diagnosis by pyrosequencing and successful treatment. Surg. Neurol. 2009;72:74–79
  12. LaBombardi VJ, Katariwala R, Pipia G. The identification of mycobacteria from solid media and directly from VersaTREK Myco bottles using the Sherlock Mycobacteria Identification HPLC system. Clin. Microbiol. Infect. 2006;12:478–481
  13. Patel JB, Leonard DG, Pan X, Musser JM, Berman RE, Nachamkin I. Sequence-based identification of Mycobacterium species using the MicroSeq 500 16S rDNA bacterial identification system. J. Clin. Microbiol. 2000;38:246–251
  14. Telenti A, Marchesi F, Balz M, Bally R, Bottger EC, Bodmer T. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J. Clin. Microbiol. 1993;31:175–178
  15. Tuohy MJ, Hall GS, Sholtis M, Procop GW. Pyrosequencing™ as a tool for the identification of common isolates of Mycobacterium sp. Diagn. Microbiol. Infect. Dis. 2005;54:245–250
  16. Vincent V, Gutierrez MC. Mycobacterium: laboratory characteristics of slowly growing mycobacteria. In: Murray PR, et al. editor. Manual of clinical microbiology. 9th ed.. Washington, DC: ASM Press; 2007;p. 573–588
  17. Yakrus MA, Hernandez SM, Floyd MM, Sikes D, Butler WR, Metchock B. Comparison of methods for identification of Mycobacterium abscessus and M. chelonae isolates. J Clin Microbiol. 2001;39:4103–4110

PII: S0732-8893(10)00078-7

doi: 10.1016/j.diagmicrobio.2010.02.022

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 3 , Pages 234-238 , July 2010

Article Tools