Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 4 , Pages 355-358, August 2010

Characterization of macrolide resistance in Mycoplasma pneumoniae isolated from children in Shanghai, China☆

  • Yang Liu

      Affiliations

    • Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
    • ,
  • Xinyu Ye

      Affiliations

    • Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
    • ,
  • Hong Zhang

      Affiliations

    • Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai 200040, P.R. China
    • ,
  • Xiaogang Xu

      Affiliations

    • Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
    • ,
  • Wanhua Li

      Affiliations

    • Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai 200040, P.R. China
    • ,
  • Demei Zhu

      Affiliations

    • Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
    • ,
  • Minggui Wang

      Affiliations

    • Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
    • Institute of Biomedical Sciences, Fudan University, Shanghai 200040, P.R. China
    • Corresponding Author InformationCorresponding author. Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, P. R. China. Tel.: +86-21-52888195; fax: +86-21-62482859.

Received 30 November 2009; accepted 12 March 2010.

Abstract 

One hundred Mycoplasma pneumoniae strains were isolated from pediatric patients from March 2008 to July 2009. Of 100 isolates, 90 (90%) were resistant to erythromycin (MICs >128 μg/mL for 88 strains and 64 μg/mL for 2 strains), azithromycin, and clarithromycin. Fluoroquinolones and tetracyclines maintain good activities against clinical M. pneumoniae isolates. Of 90 macrolide-resistant M. pneumoniae strains, 88 (98%) harbored an A-to-G transition mutation at position 2063 in 23S rRNA genes, and the remaining 2 showed either A2064G or A2063T mutation; the latter point mutation is newly discovered and reported. Ninety-three (93%) clinical isolates were classified into the P1 gene restriction fragment length polymorphism (RFLP) type I, and 7 (7%) were type II.

Keywords: Mycoplasma pneumoniae, Antimicrobial susceptibility, Macrolides, 23S rRNA, PCR-RFLP

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☆ This article was presented in part by slides presentation and got the Young Investigator Award at the eighth National Conference on Infectious Diseases and Antimicrobial Chemotherapy, Shanghai, China, September 23 to 26, 2009.

PII: S0732-8893(10)00088-X

doi:10.1016/j.diagmicrobio.2010.03.004

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 4 , Pages 355-358, August 2010

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