Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 4 , Pages 333-336, August 2010

Comparison of real-time polymerase chain reaction and conventional biochemical methods for identification of Mycobacterium chelonae–Mycobacterium abscessus group to the species level

  • Nora Guarin

      Affiliations

    • Clinical Microbiology Laboratory, Stanford Hospital and Clinics, Palo Alto, CA 94304, USA
    • ,
  • Indre Budvytiene

      Affiliations

    • Clinical Microbiology Laboratory, Stanford Hospital and Clinics, Palo Alto, CA 94304, USA
    • ,
  • Laleh Ghafghaichi

      Affiliations

    • Clinical Microbiology Laboratory, Stanford Hospital and Clinics, Palo Alto, CA 94304, USA
    • ,
  • Niaz Banaei

      Affiliations

    • Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    • Department of Medicine (Infectious Diseases), Stanford University School of Medicine, Stanford, CA 94305, USA
    • Clinical Microbiology Laboratory, Stanford Hospital and Clinics, Palo Alto, CA 94304, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-650-736-8052; fax: +1-650-725-5671.

Received 7 February 2010; accepted 20 March 2010.

Abstract 

The Mycobacterium chelonae–Mycobacterium abscessus group (MCAG) is the most common cause of infections because of rapidly growing mycobacteria. Rapid identification of MCAG to the species level is essential for choosing empiric antibiotic treatment and for public health measures. In this study, we compared the performance of a single-tube multiplex, real-time polymerase chain reaction (PCR) assay to 3 biochemical tests for species-level identification of 46 MCAG isolates. We show that real-time PCR provides the most accurate results for rapid species-level identification of MCAG.

Keywords: M. chelonae–M. abscessus group, M. immunogenum, Real-time PCR, Identification

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PII: S0732-8893(10)00117-3

doi:10.1016/j.diagmicrobio.2010.03.011

Diagnostic Microbiology & Infectious Disease
Volume 67, Issue 4 , Pages 333-336, August 2010

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