Diagnostic Microbiology & Infectious Disease
Volume 72, Issue 2 , Pages 131-138, February 2012

Pyrosequencing-based validation of a simple cell-suspension polymerase chain reaction assay for Campylobacter with application of high-processivity polymerase and novel internal amplification controls for rapid and specific detection☆☆☆

  • Brian B. Oakley

      Affiliations

    • Poultry Microbiological Safety Research Unit, USDA Agricultural Research Service, Richard B. Russell Agricultural Research Center, Athens, GA 30605, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-706-546-3166; fax: +1-706-546-3771.
    • ,
  • J. Eric Line

      Affiliations

    • Poultry Microbiological Safety Research Unit, USDA Agricultural Research Service, Richard B. Russell Agricultural Research Center, Athens, GA 30605, USA
    • ,
  • Mark E. Berrang

      Affiliations

    • Bacterial Epidemiology and Antibiotic Resistance Unit, USDA Agricultural Research Service, Richard B. Russell Agricultural Research Center, Athens, GA 30605, USA
    • ,
  • Jessica M. Johnson

      Affiliations

    • South Carolina State University, Orangeburg, SC 29117, USA
    • ,
  • R. Jeff Buhr

      Affiliations

    • Poultry Microbiological Safety Research Unit, USDA Agricultural Research Service, Richard B. Russell Agricultural Research Center, Athens, GA 30605, USA
    • ,
  • Nelson A. Cox

      Affiliations

    • Poultry Microbiological Safety Research Unit, USDA Agricultural Research Service, Richard B. Russell Agricultural Research Center, Athens, GA 30605, USA
    • ,
  • Kelli L. Hiett

      Affiliations

    • Poultry Microbiological Safety Research Unit, USDA Agricultural Research Service, Richard B. Russell Agricultural Research Center, Athens, GA 30605, USA
    • ,
  • Bruce S. Seal

      Affiliations

    • Poultry Microbiological Safety Research Unit, USDA Agricultural Research Service, Richard B. Russell Agricultural Research Center, Athens, GA 30605, USA

Received 23 August 2011; accepted 3 November 2011.

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☆ Funding was provided by the ARS, USDA CRIS project 6612-32000-055 Molecular Characterization and Gastrointestinal Tract Ecology of Commensal Human Food-Borne Bacterial Pathogens in the Chicken.

☆☆ Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

PII: S0732-8893(11)00448-2

doi:10.1016/j.diagmicrobio.2011.11.001

Diagnostic Microbiology & Infectious Disease
Volume 72, Issue 2 , Pages 131-138, February 2012

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