Virology
Rapid molecular diagnostic test for Zika virus with low demands on sample preparation and instrumentation

https://doi.org/10.1016/j.diagmicrobio.2016.08.027Get rights and content
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Highlights

  • An isothermal nucleic acid amplification method for the rapid detection of Zika virus

  • The method displayed high levels of sensitivity and specificity for Zika virus RNA

  • The method is compatible with crude sample preparation and reactions can be performed on a portable device

  • This could be applied for early detection of outbreaks or on-site pathogen detection.

Abstract

Zika virus has only recently gained attention due to recent large outbreaks worldwide. An easy to use nucleic acid amplification test could play an important role in the early detection of the infection and patient management. Here, we report a rapid and robust isothermal nucleic acid amplification assay for the detection of Zika virus. The method is cost-effective and compatible with portable instrumentation, enabling near patient testing and field use.

Keywords

Zika
Virus
Diagnostics
RNA
Isothermal
Amplification
Portable
Outbreak

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