VirologyEvaluation of a commercially available Zika virus IgM ELISA: specificity in focus
Section snippets
Financial support
This study was supported by Cadham Provincial Laboratory internal research funds.
Conflict of interest
None Declared.
Acknowledgement
We would like to thank the Viral Zoonoses section at National Microbiology Laboratory in Winnipeg, Canada for their kind help.
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2020, Journal of InfectionClinical and neurodevelopmental features in children with cerebral palsy and probable congenital Zika
2019, Brain and DevelopmentCitation Excerpt :Arthrogryposis was present in a slight less extent than described by previous series, that showed a range from 10.0% [22] to 27.3% [23]. Laboratorial evidence of the congenital Zika virus infection (i.e., positive IgM or RT-PCR) was seen in only 8 (9.7%) children and serological evidence of previous contact with ZIKV was noted in 48.8%, meaning that the majority (58.5%) had some laboratory evidence, even though the serological test has been reported to have a low sensitivity [24]. Also, there have been a few descriptions showing individuals with no seroconversion after the acute phase of a ZIKV infection [25] and the case of a typical CZS child with a positive RT-PCR in amniotic fluid, but whose postnatal RT-PCR and IgM were negative [26].
Added value of IgA antibodies against Zika virus non-structural protein 1 in the diagnosis of acute Zika virus infections
2019, Journal of Virological MethodsCitation Excerpt :Five of the seven patients with a positive IgA result also tested positive by anti-ZIKV-NS1 IgG ELISA and/or FFNT (data not shown), providing evidence for a recent co-infection. In regards to the NS1-based ZIKV IgM ELISA, several studies have also demonstrated a high specificity (≥98.7) using panels of potentially cross-reactive specimens and/or healthy controls (Borena et al., 2017; Huzly et al., 2016; Kadkhoda et al., 2017; Steinhagen et al., 2016a), while others reported elevated IgM cross-reactivity with DENV-positive specimens (L’Huillier et al., 2017). The strengths of this study include the use of well-characterised, RT-PCR-confirmed ZIKV cases.