Virology
Evaluation of a commercially available Zika virus IgM ELISA: specificity in focus

https://doi.org/10.1016/j.diagmicrobio.2017.04.002Get rights and content

Abstract

Zika virus (ZIKV), an emerging arbovirus, has become the center of attention since it was linked to congenital abnormalities. Given the overlapping clinical picture of ZIKV disease with other infectious diseases and as an exigent public health matter, timely and reliable diagnosis of ZIKV infection is crucial. We evaluated the performance of a commercially available NS1-based ZIKV IgM ELISA. Our results showed suboptimal sensitivity within the first 5 days after onset while specificity remained robust.

Section snippets

Financial support

This study was supported by Cadham Provincial Laboratory internal research funds.

Conflict of interest

None Declared.

Acknowledgement

We would like to thank the Viral Zoonoses section at National Microbiology Laboratory in Winnipeg, Canada for their kind help.

References (21)

There are more references available in the full text version of this article.

Cited by (28)

  • Validation of Zika virus infections: Nonmolecular aspects, immunoassays, and beyond

    2021, Zika Virus Biology, Transmission, and Pathways: Volume 1: The Neuroscience of Zika Virus
  • Clinical and neurodevelopmental features in children with cerebral palsy and probable congenital Zika

    2019, Brain and Development
    Citation Excerpt :

    Arthrogryposis was present in a slight less extent than described by previous series, that showed a range from 10.0% [22] to 27.3% [23]. Laboratorial evidence of the congenital Zika virus infection (i.e., positive IgM or RT-PCR) was seen in only 8 (9.7%) children and serological evidence of previous contact with ZIKV was noted in 48.8%, meaning that the majority (58.5%) had some laboratory evidence, even though the serological test has been reported to have a low sensitivity [24]. Also, there have been a few descriptions showing individuals with no seroconversion after the acute phase of a ZIKV infection [25] and the case of a typical CZS child with a positive RT-PCR in amniotic fluid, but whose postnatal RT-PCR and IgM were negative [26].

  • Added value of IgA antibodies against Zika virus non-structural protein 1 in the diagnosis of acute Zika virus infections

    2019, Journal of Virological Methods
    Citation Excerpt :

    Five of the seven patients with a positive IgA result also tested positive by anti-ZIKV-NS1 IgG ELISA and/or FFNT (data not shown), providing evidence for a recent co-infection. In regards to the NS1-based ZIKV IgM ELISA, several studies have also demonstrated a high specificity (≥98.7) using panels of potentially cross-reactive specimens and/or healthy controls (Borena et al., 2017; Huzly et al., 2016; Kadkhoda et al., 2017; Steinhagen et al., 2016a), while others reported elevated IgM cross-reactivity with DENV-positive specimens (L’Huillier et al., 2017). The strengths of this study include the use of well-characterised, RT-PCR-confirmed ZIKV cases.

View all citing articles on Scopus
View full text