Bacteriology
A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs

https://doi.org/10.1016/j.diagmicrobio.2017.08.007Get rights and content

Highlights

  • Highly sensitive and specific Chlamydia trachomatis assay is reported.

  • Utilizes filtration isolation of nucleic acids (FINA) sample prep method.

  • Assay performance was validated with 40 clinical swab specimens.

Abstract

A highly sensitive and specific Chlamydia trachomatis (CT) diagnostic test was developed by combining filtration isolation of nucleic acid (FINA) extraction with quantitative polymerase chain reaction including an internal control to identify test inhibition. A pilot study of 40 clinical specimens yielded 100% sensitivity and specificity.

Section snippets

Acknowledgements

We thank Johns Hopkins Center for STD Point-of-Care Tests for providing the Chlamydia trachomatis swab specimens and microbial DNA for the specificity panel, and special thanks to Justin Hardick for providing us with quantified CT DNA to standardize the CT-infected McCoy cell preps. MAB and SMM were supported by SP0030079 and SP0035724 JHU POCT, Johns Hopkins Center for STD Point-of-Care Tests. The sponsor had no role in study design or collection, analysis or interpretation of data presented

References (13)

There are more references available in the full text version of this article.

Cited by (0)

View full text